BUB1/YGR188C Summary Help

BUB1 BASIC INFORMATION

Standard Name BUB1
Systematic Name YGR188C
Feature Type ORF, Verified
Description Protein kinase that forms a complex with Mad1p and Bub3p that is crucial in the checkpoint mechanism required to prevent cell cycle progression into anaphase in the presence of spindle damage, associates with centromere DNA via Skp1p (1, 2, 3, 4 and see Summary Paragraph)
Name Description Budding Uninhibited by Benzimidazole 5
GO Annotations All BUB1 GO evidence and references
    View Computational GO annotations for BUB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All BUB1 Phenotype details and references
Classical genetics
null
reduction of function
Large-scale survey
null
Interactions BUB1 All interactions details and references
304 total interaction(s) for 205 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 1
  • Co-localization: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 14
Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 151
  • Phenotypic Suppression: 51
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 48
  • Synthetic Rescue: 4
Sequence Information
ChrVII:875114 to 872049 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 150 cM
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3066 875114..872049 2004-07-20 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003420

BUB1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Literature
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  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BUB1

SUMMARY PARAGRAPH for BUB1

BUB1 encodes a non-essential protein kinase involved in the spindle checkpoint (2). The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules (see 6 for review; reference 7 describes defects detected by the checkpoint). The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators (6). Bub1p binds to and phosphorylates another spindle checkpoint protein, Bub3p, and autophosphorylates (2). Like other spindle checkpoint mutants, bub1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization (5). Overexpression of a dominant mutant, BUB1-5, delays mitosis, as does overexpression of MPS1, which encodes another protein kinase involved in the spindle checkpoint (1, 8). The BUB1-5 mitotic delay requires other spindle checkpoint components including Mps1p, Bub2p, Bub3p, Mad1p, Mad2p, and Mad3p (1). Bub1p, Bub3p, Mad1p, Mad2p, Mad3p, and Mps1p act in a branch of the spindle checkpoint pathway that may prevent premature chromosome disjunction. A second branch involves Bub2p and Bfa1p, and may prevent cytokinesis prior to chromosome segregation (9, 10, 11, 12). Mad3p has sequence similarity to Bub1p, but the similaroty does not include the kinase domain of Bub1p (13). Protein kinases related to Bub1p are involved in checkpoint control and timing of mitosis in several other species, including S. pombe (14), mouse (15), and human (16). Mutations in the human BUB1 homologs (BUB1, BUB1B) are associated with several types of cancer (17, 18).

Last updated: 2005-07-01

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BUB1]

1) Farr KA and Hoyt MA  (1998) Bub1p kinase activates the Saccharomyces cerevisiae spindle assembly checkpoint. Mol Cell Biol 18(5):2738-47
2) Roberts BT, et al.  (1994) The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol Cell Biol 14(12):8282-91
3) Brady DM and Hardwick KG  (2000) Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function. Curr Biol 10(11):675-8
4) Kitagawa K, et al.  (2003) Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint. Mol Cell 11(5):1201-13
5) Hoyt MA, et al.  (1991) S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell 66(3):507-17
6) Hardwick KG  (1998) The spindle checkpoint. Trends Genet 14(1):1-4
7) Hardwick KG, et al.  (1999) Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Genetics 152(2):509-18
8) Hardwick KG, et al.  (1996) Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption. Science 273(5277):953-6
9) Alexandru G, et al.  (1999) Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J 18(10):2707-21
10) Fesquet D, et al.  (1999) A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast. EMBO J 18(9):2424-34
11) Fraschini R, et al.  (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145(5):979-91
12) Li R  (1999) Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A 96(9):4989-94
13) Hardwick KG, et al.  (2000) MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p. J Cell Biol 148(5):871-82
14) Bernard P, et al.  (1998) Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis. J Cell Biol 143(7):1775-87
15) Taylor SS and McKeon F  (1997) Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to spindle damage. Cell 89(5):727-35
16) Ouyang B, et al.  (1998) Human Bub1: a putative spindle checkpoint kinase closely linked to cell proliferation. Cell Growth Differ 9(10):877-85
17) Cahill DP, et al.  (1998) Mutations of mitotic checkpoint genes in human cancers. Nature 392(6673):300-3
18) Yamaguchi K, et al.  (1999) Mutation analysis of hBUB1 in aneuploid HNSCC and lung cancer cell lines. Cancer Lett 139(2):183-7