RNR4/YGR180C Summary

RNR4 BASIC INFORMATION

Standard Name	RNR4 1, 2
Systematic Name	YGR180C
Alias	CRT3 3 , PSO3 4 , 5 , 6
Feature Type	ORF, Verified
Description	Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits (7)
Name Description	RiboNucleotide Reductase 2

GO Annotations	All RNR4 GO evidence and references
	View Computational GO annotations for RNR4
Molecular Function
Manually curated	contributes_to ribonucleoside-diphosphate reductase activity (IDA, IMP) ribonucleoside-diphosphate reductase activity (IGI, IMP)
Biological Process
Manually curated	cofactor biosynthetic process (IMP, IPI) deoxyribonucleotide biosynthetic process (IDA)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA) ribonucleoside-diphosphate reductase complex (IDA, IPI)

Pathways	de novo biosynthesis of purine nucleotides de novo biosynthesis of pyrimidine deoxyribonucleotides superpathway of histidine, purine, and pyrimidine biosynthesis superpathway of purine biosynthesis and salvage pathways

Mutant Phenotype	All RNR4 Phenotype details and references
Classical genetics
null	budding index: increased cell cycle progression: abnormal cell cycle progression in S phase: arrested cell size: increased cold sensitivity: increased Rnr1p accumulation: increased Rnr2p accumulation: increased Rnr3p accumulation: increased resistance to hydroxyurea: decreased resistance to benomyl: increased resistance to stannous chloride: decreased vegetative growth: decreased viable
unspecified	chromosome/plasmid maintenance: abnormal mitochondrial rho- mutation frequency: increased mutation frequency: decreased mutation frequency: increased resistance to 8-methoxypsoralen: decreased resistance to 3-carbethoxypsoralen: decreased resistance to hydrogen peroxide: decreased resistance to paraquat: decreased resistance to bleomycin A2: decreased viability: decreased
Large-scale survey
null	acid pH resistance: decreased bud morphology: abnormal competitive fitness: decreased resistance to rapamycin: decreased resistance to wortmannin: increased resistance to L-1,4-dithiothreitol: decreased resistance to hydroxyurea: increased resistance to doxorubicin: decreased respiratory growth: decreased rate respiratory growth: absent toxin resistance: decreased transposable element transposition: increased utilization of carbon source: decreased viable

Interactions	RNR4 All interactions details and references
	89 total interaction(s) for 61 unique genes/features.
Physical Interactions	Affinity Capture-MS: 17 Affinity Capture-RNA: 1 Affinity Capture-Western: 7 Biochemical Activity: 3 Co-crystal Structure: 2 Co-localization: 3 Co-purification: 2 Reconstituted Complex: 6 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 2 Phenotypic Enhancement: 3 Phenotypic Suppression: 3 Synthetic Growth Defect: 10 Synthetic Lethality: 28

Sequence Information

ChrVII:856306 to 855269 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-07-20 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1038	856306..855269	2004-07-20	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003412

RNR4 RESOURCES

Click on map for expanded view

SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for RNR4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RNR4]

1)	Huang M and Elledge SJ (1997) Identification of RNR4, encoding a second essential small subunit of ribonucleotide reductase in Saccharomyces cerevisiae. Mol Cell Biol 17(10):6105-13
2)	Wang PJ, et al. (1997) Rnr4p, a novel ribonucleotide reductase small-subunit protein. Mol Cell Biol 17(10):6114-21
3)	Zhou Z and Elledge SJ (1992) Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131(4):851-66
4)	Henriques JA and Moustacchi E (1980) Isolation and characterization of pso mutants sensitive to photo-addition of psoralen derivatives in Saccharomyces cerevisiae. Genetics 95(2):273-88
5)	Brendel M, et al. (2003) Role of PSO genes in repair of DNA damage of Saccharomyces cerevisiae. Mutat Res 544(2-3):179-93
6)	Strauss M, et al. (2007) RNR4 mutant alleles pso3-1 and rnr4Delta block induced mutation in Saccharomyces cerevisiae. Curr Genet 51(4):221-31
7)	Yao R, et al. (2003) Subcellular localization of yeast ribonucleotide reductase regulated by the DNA replication and damage checkpoint pathways. Proc Natl Acad Sci U S A 100(11):6628-33

SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.