TRS65/YGR166W Summary Help

Standard Name TRS65 1
Systematic Name YGR166W
Alias KRE11 2
Feature Type ORF, Verified
Description Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; role in cell wall beta-glucan biosynthesis and the stress response (1, 2, 3, 4, 5)
Name Description TRapp Subunit 1
Chromosomal Location
ChrVII:830515 to 832197 | ORF Map | GBrowse
Gbrowse
Genetic position: 154.34 cM
Gene Ontology Annotations All TRS65 GO evidence and references
  View Computational GO annotations for TRS65
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
252 total interaction(s) for 141 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 14
  • Co-fractionation: 1
  • Protein-RNA: 1
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 117
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 6
  • Positive Genetic: 8
  • Synthetic Growth Defect: 31
  • Synthetic Lethality: 26

Resources
Expression Summary
histogram
Resources
Length (a.a.) 560
Molecular Weight (Da) 63,367
Isoelectric Point (pI) 9.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:830515 to 832197 | ORF Map | GBrowse
SGD ORF map
Genetic position: 154.34 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1683 830515..832197 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003398
References cited on this page View Complete Literature Guide for TRS65
1) Sacher M, et al.  (2000) Identification and characterization of five new subunits of TRAPP. Eur J Cell Biol 79(2):71-80
2) Brown JL, et al.  (1993) A mutational analysis of killer toxin resistance in Saccharomyces cerevisiae identifies new genes involved in cell wall (1-->6)-beta-glucan synthesis. Genetics 133(4):837-49
3) Sacher M, et al.  (2001) TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport. Mol Cell 7(2):433-42
4) Fernandez-Ricaud L, et al.  (2007) PROPHECY--a yeast phenome database, update 2006. Nucleic Acids Res 35(Database issue):D463-7
5) Liang Y, et al.  (2007) The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex. Mol Biol Cell 18(7):2533-41