TIF4631/YGR162W Summary Help

Standard Name TIF4631 1, 2
Systematic Name YGR162W
Feature Type ORF, Verified
Description Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication (1, 3, 4, 5, 6)
Name Description Translation Initiation Factor 1, 2
Gene Product Alias eiF4G1 7
Chromosomal Location
ChrVII:824059 to 826917 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TIF4631 GO evidence and references
  View Computational GO annotations for TIF4631
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
conditional
null
overexpression
Large-scale survey
null
overexpression
Resources
267 total interaction(s) for 143 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 139
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 31
  • Co-crystal Structure: 2
  • Far Western: 1
  • PCA: 1
  • Reconstituted Complex: 28
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 18
  • Positive Genetic: 25
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 8
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 952
Molecular Weight (Da) 107,101
Isoelectric Point (pI) 5.91
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:824059 to 826917 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2859 824059..826917 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003394
References cited on this page View Complete Literature Guide for TIF4631
1) Goyer C, et al.  (1993) TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function. Mol Cell Biol 13(8):4860-74
2) Lanker S, et al.  (1992) Interactions of the eIF-4F subunits in the yeast Saccharomyces cerevisiae. J Biol Chem 267(29):21167-71
3) Tarun SZ Jr, et al.  (1997) Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation. Proc Natl Acad Sci U S A 94(17):9046-51
4) Dominguez D, et al.  (1999) Interaction of translation initiation factor eIF4G with eIF4A in the yeast Saccharomyces cerevisiae. J Biol Chem 274(38):26720-6
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Li Z, et al.  (2009) Rational extension of the ribosome biogenesis pathway using network-guided genetics. PLoS Biol 7(10):e1000213
7) Neff CL and Sachs AB  (1999) Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally. Mol Cell Biol 19(8):5557-64