TIF4631/YGR162W Summary

TIF4631/YGR162W Summary

Standard Name	TIF4631 1, 2
Systematic Name	YGR162W
Feature Type	ORF, Verified
Description	Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication (1, 3, 4, 5, 6)
Name Description	Translation Initiation Factor 1, 2
Gene Product Alias	eiF4G1 7

Chromosomal Location	ChrVII:824059 to 826917 \| ORF Map \| GBrowse

Gene Ontology Annotations All TIF4631 GO evidence and references

	View Computational GO annotations for TIF4631
Molecular Function
Manually curated	translation initiation factor activity (TAS)
Biological Process
Manually curated	ribosomal large subunit biogenesis (IMP) stress granule assembly (IMP) translational initiation (TAS)
Cellular Component
Manually curated	cytoplasmic stress granule (IDA) eukaryotic translation initiation factor 4F complex (IGI, IMP, ISS) ribosome (TAS)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All TIF4631 Phenotype evidence and references

Classical genetics
conditional	cold sensitivity: increased
null	cold sensitivity: increased nuclear export: decreased 60S ribosomal subunit accumulation: decreased resistance to methyl methanesulfonate: increased resistance to tunicamycin: increased viable
Large-scale survey
null	auxotrophy bud morphology: abnormal cell cycle progression in G1 phase: delayed glycogen accumulation: increased competitive fitness: decreased dessication resistance: decreased fermentative growth: decreased rate haploinsufficient heat sensitivity: increased ionic stress resistance: decreased replicative lifespan: increased resistance to St. John's Wort extract: decreased resistance to sirolimus: decreased resistance to hygromycin B: decreased resistance to benomyl: decreased resistance to doxorubicin: decreased sporulation: decreased sporulation: absent stress resistance: increased toxin resistance: decreased vegetative growth: decreased rate
overexpression	cell cycle progression: abnormal vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All TIF4631 Interaction evidence and references

	261 total interaction(s) for 141 unique genes/features.
Physical Interactions	Affinity Capture-MS: 137 Affinity Capture-RNA: 1 Affinity Capture-Western: 33 Co-crystal Structure: 2 Far Western: 1 PCA: 1 Reconstituted Complex: 24 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 2 Negative Genetic: 18 Positive Genetic: 24 Synthetic Growth Defect: 4 Synthetic Lethality: 8 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All TIF4631 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:824059 to 826917 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2859	824059..826917	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003394

References cited on this page View Complete Literature Guide for TIF4631

1)	Goyer C, et al. (1993) TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function. Mol Cell Biol 13(8):4860-74
2)	Lanker S, et al. (1992) Interactions of the eIF-4F subunits in the yeast Saccharomyces cerevisiae. J Biol Chem 267(29):21167-71
3)	Tarun SZ Jr, et al. (1997) Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation. Proc Natl Acad Sci U S A 94(17):9046-51
4)	Dominguez D, et al. (1999) Interaction of translation initiation factor eIF4G with eIF4A in the yeast Saccharomyces cerevisiae. J Biol Chem 274(38):26720-6
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Li Z, et al. (2009) Rational extension of the ribosome biogenesis pathway using network-guided genetics. PLoS Biol 7(10):e1000213
7)	Neff CL and Sachs AB (1999) Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally. Mol Cell Biol 19(8):5557-64