MTR3/YGR158C Summary 
MTR3 BASIC INFORMATION
| Standard Name | MTR3 1 |
|---|---|
| Systematic Name | YGR158C |
| Feature Type | ORF, Verified |
| Description | Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) (2, 3, 4, 5 and see Summary Paragraph) 
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| Name Description | MRNA TRansport 6 |
| Interactions | MTR3 All interactions details and references |
|---|---|
| 88 total interaction(s) for 22 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000003390 |
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MTR3 RESOURCES
| SGD ORF map | GBrowse |
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for MTR3
SUMMARY PARAGRAPH for MTR3
The exosome complex possesses 3'-5' exonuclease and endoribonucleolytic activities that are essential for diverse ribonucleolytic processes in both the nucleus and the cytoplasm (7, 8, 9). The nuclear exosome is associated with the TRAMP complex and is involved in RNA catabolic processes including RNA surveillance (10, 11 and references therein), pre-mRNA turnover (12) and the production of mature 3' ends for snoRNAs, snRNAs and rRNAs (8, 13 and references therein). The cytoplasmic exosome is associated with Ski7p and the SKI complex and is involved in RNA catabolic processes that include both the routine turnover of normal mRNA (14) as well as the degradation of aberrant mRNAs (15 and references therein). The 10-subunit core exosome complex (Csl4p, Rrp4p, Rrp40p, Ski6p, Rrp42p, Rrp43p, Rrp45p, Rrp46p, Mtr3p, Dis3p) is the same in both locations, but the nuclear exosome contains an additional subunit (Rrp6p) and two additional accessory factors (Lrp1p, Mpp6p) (9).
Although the exosome was originally described as a "complex of exonucleases," with multiple subunits proposed to have RNase activity (7), later work has shown that this mechanism is unlikely in yeast. With the exception of Ski6p, none of the yeast subunits that show homology to E. coli RNase PH retain the active site residues seen in the bacterial or archael enzymes. Further research has also demonstrated that most, if not all, detectable enzymatic activity resides in the Dis3p and Rrp6p subunits (4, 5).
MTR3 encodes a core subunit of the exosome and is predicted to be a member of the RNase PH class of RNases (2, 3 and references therein). Like most exosome components, Mtr3p is highly conserved among eukaryotes, including humans (hMtr3p (EXOSC6)) (4 and references therein). MTR3 is an essential gene, but a temperature sensitive mutant (at the restrictive temperature) accumulates aberrant forms of snoRNA, snRNA (8), and rRNA (2, 13, 16). This same mutant also accumulates nucleolar polyA+ mRNA, a phenotype with led to the original description of MTR3 as a gene involved in mRNA transport (1).
Last updated: 2009-09-09
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MTR3]
| 1) | Kadowaki T, et al. (1995) Mutations in nucleolar proteins lead to nucleolar accumulation of polyA+ RNA in Saccharomyces cerevisiae. Mol Biol Cell 6(9):1103-10 |
| 2) | Allmang C, et al. (1999) The yeast exosome and human PM-Scl are related complexes of 3' --> 5' exonucleases. Genes Dev 13(16):2148-58 |
| 3) | Synowsky SA, et al. (2006) Probing genuine strong interactions and post-translational modifications in the heterogeneous yeast exosome protein complex. Mol Cell Proteomics 5(9):1581-92 |
| 4) | Liu Q, et al. (2006) Reconstitution, activities, and structure of the eukaryotic RNA exosome. Cell 127(6):1223-37 |
| 5) | Dziembowski A, et al. (2007) A single subunit, Dis3, is essentially responsible for yeast exosome core activity. Nat Struct Mol Biol 14(1):15-22 |
| 6) | Kadowaki T, et al. (1994) Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants. J Cell Biol 126(3):649-59 |
| 7) | Mitchell P, et al. (1997) The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases. Cell 91(4):457-66 |
| 8) | van Hoof A, et al. (2000) Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs. Mol Cell Biol 20(2):441-52 |
| 9) | Synowsky SA, et al. (2009) Comparative multiplexed mass spectrometric analyses of endogenously expressed yeast nuclear and cytoplasmic exosomes. J Mol Biol 385(4):1300-13 |
| 10) | Vanacova S, et al. (2005) A new yeast poly(A) polymerase complex involved in RNA quality control. PLoS Biol 3(6):e189 |
| 11) | LaCava J, et al. (2005) RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. Cell 121(5):713-24 |
| 12) | Bousquet-Antonelli C, et al. (2000) Identification of a regulated pathway for nuclear pre-mRNA turnover. Cell 102(6):765-75 |
| 13) | Allmang C, et al. (2000) Degradation of ribosomal RNA precursors by the exosome. Nucleic Acids Res 28(8):1684-91 |
| 14) | Anderson JS and Parker RP (1998) The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex. EMBO J 17(5):1497-506 |
| 15) | Schaeffer D, et al. (2008) Determining in vivo activity of the yeast cytoplasmic exosome. Methods Enzymol 448:227-39 |
| 16) | Houseley J, et al. (2007) Trf4 targets ncRNAs from telomeric and rDNA spacer regions and functions in rDNA copy number control. EMBO J 26(24):4996-5006 |
