CCM1/YGR150C Summary

CCM1/YGR150C Summary

Standard Name	CCM1 1
Systematic Name	YGR150C
Alias	RRG2 2 , DMR1 3
Feature Type	ORF, Verified
Description	Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport (1, 3, 4, 5, 6, 7, 8)
Name Description	COB and COX1 mRNA maturation 1

Chromosomal Location	ChrVII:793053 to 790459 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All CCM1 GO evidence and references

	View Computational GO annotations for CCM1
Molecular Function
Manually curated	rRNA binding (IDA)
Biological Process
Manually curated	mitochondrial genome maintenance (IMP) mitochondrial RNA processing (IMP) rRNA metabolic process (IMP)
Cellular Component
Manually curated	mitochondrion (IDA)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All CCM1 Phenotype evidence and references

Classical genetics
null	15S rRNA accumulation: decreased COX1 mRNA modification: abnormal COB mRNA modification: abnormal metal resistance: increased mitochondrial genome maintenance: abnormal petite respiratory growth: absent
Large-scale survey
null	acid pH resistance: decreased cell size: decreased glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased hyperosmotic stress resistance: decreased mitochondrial morphology: abnormal resistance to pyocyanin: increased resistance to 2,4-dichlorophenol: decreased resistance to nickel sulfate: decreased resistance to ethanol: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased respiratory growth: decreased rate respiratory growth: decreased respiratory growth: absent stress resistance: increased utilization of carbon source: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All CCM1 Interaction evidence and references

	30 total interaction(s) for 30 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 2 Two-hybrid: 17
Genetic Interactions	Negative Genetic: 4 Synthetic Growth Defect: 3 Synthetic Lethality: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All CCM1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:793053 to 790459 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2595	793053..790459	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003382

References cited on this page View Complete Literature Guide for CCM1

1)	Moreno JI, et al. (2009) Ccm1p/Ygr150cp, a pentatricopeptide repeat protein, is essential to remove the fourth intron of both COB and COX1 pre-mRNAs in Saccharomyces cerevisiae. Curr Genet 55(4):475-84
2)	Merz S and Westermann B (2009) Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae. Genome Biol 10(9):R95
3)	Puchta O, et al. (2010) DMR1 (CCM1/YGR150C) of Saccharomyces cerevisiae encodes an RNA-binding protein from the pentatricopeptide repeat family required for the maintenance of the mitochondrial 15S ribosomal RNA. Genetics 184(4):959-73
4)	Dimmer KS, et al. (2002) Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae. Mol Biol Cell 13(3):847-53
5)	Steinmetz LM, et al. (2002) Systematic screen for human disease genes in yeast. Nat Genet 31(4):400-4
6)	Ellis TP, et al. (2004) Aep3p stabilizes the mitochondrial bicistronic mRNA encoding subunits 6 and 8 of the H+-translocating ATP synthase of Saccharomyces cerevisiae. J Biol Chem 279(16):15728-33
7)	Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
8)	Herst PM, et al. (2008) Plasma membrane electron transport in Saccharomyces cerevisiae depends on the presence of mitochondrial respiratory subunits. FEMS Yeast Res 8(6):897-905