THI4/YGR144W Summary Help

Standard Name THI4 1
Systematic Name YGR144W
Alias ESP35 , MOL1 2
Feature Type ORF, Verified
Description Thiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents (1, 3, 4, 5, 6)
Name Description THIamine metabolism
Chromosomal Location
ChrVII:780399 to 781379 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All THI4 GO evidence and references
  View Computational GO annotations for THI4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Pathways
Classical genetics
null
unspecified
Large-scale survey
null
Resources
16 total interaction(s) for 13 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Negative Genetic: 2
  • Positive Genetic: 2
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 326
Molecular Weight (Da) 34,991
Isoelectric Point (pI) 6.23
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:780399 to 781379 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..981 780399..781379 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003376
References cited on this page View Complete Literature Guide for THI4
1) Praekelt UM, et al.  (1994) Regulation of THI4 (MOL1), a thiamine-biosynthetic gene of Saccharomyces cerevisiae. Yeast 10(4):481-90
2) Praekelt UM and Meacock PA  (1992) MOL1, a Saccharomyces cerevisiae gene that is highly expressed in early stationary phase during growth on molasses. Yeast 8(9):699-710
3) Machado CR, et al.  (1997) Dual role for the yeast THI4 gene in thiamine biosynthesis and DNA damage tolerance. J Mol Biol 273(1):114-21
4) Chatterjee A, et al.  (2007) Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate. J Am Chem Soc 129(10):2914-22
5) Chatterjee A, et al.  (2008) Biosynthesis of the thiamin-thiazole in eukaryotes: identification of a thiazole tautomer intermediate. J Am Chem Soc 130(34):11394-8
6) Chatterjee A, et al.  (2011) Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase. Nature 478(7370):542-6