CBF2/YGR140W Summary Help

CBF2 BASIC INFORMATION

Standard Name CBF2
Systematic Name YGR140W
Alias CEP2 , CTF14 1 , NDC10 , CSL5 2
Feature Type ORF, Verified
Description Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo (3, 4, 5)
Also known as: CBF3A
Name Description Centromere-Binding Factor 6
GO Annotations All CBF2 GO evidence and references
    View Computational GO annotations for CBF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All CBF2 Phenotype details and references
Classical genetics
repressible
Large-scale survey
null
Interactions CBF2 All interactions details and references
104 total interaction(s) for 68 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 7
  • Biochemical Activity: 4
  • Co-localization: 8
  • Protein-peptide: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 22
Genetic Interactions
  • Dosage Lethality: 10
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 10
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 9
  • Synthetic Rescue: 3
Sequence Information
ChrVII:767434 to 770304 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2871 767434..770304 2004-07-20 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003372

CBF2 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for CBF2

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
VMA5Nomenclature conflictCSL5 has been used in the literature to refer to both CBF2/YGR140W (Baker, RE. et al. (1998) Genetics 149:73; PMID:9584087), which encodes a centromere binding factor (CBF3a), and VMA5/YKL080W (White, WH. and Johnson, DI. (1997) Genetics 147:43; PMID: 9286667), which encodes subunit C of a vacuolar H(+)-ATPase.

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CBF2]

1) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
2) Baker RE, et al.  (1998) Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics 149(1):73-85
3) Lechner J and Carbon J  (1991) A 240 kd multisubunit protein complex, CBF3, is a major component of the budding yeast centromere. Cell 64(4):717-25
4) Yoon HJ and Carbon J  (1995) Genetic and biochemical interactions between an essential kinetochore protein, Cbf2p/Ndc10p, and the CDC34 ubiquitin-conjugating enzyme. Mol Cell Biol 15(9):4835-42
5) Espelin CW, et al.  (2003) Binding of the essential Saccharomyces cerevisiae kinetochore protein Ndc10p to CDEII. Mol Biol Cell 14(11):4557-68
6) Jiang W, et al.  (1993) Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore. J Cell Biol 121(3):513-9