Standard Name	LSB1 1
Systematic Name	YGR136W
Feature Type	ORF, Verified
Description	Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description	Las Seventeen Binding protein 1

Chromosomal Location	ChrVII:762428 to 763153 | ORF Map | GBrowse

	View Computational GO annotations for LSB1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	negative regulation of Arp2/3 complex-mediated actin nucleation (IDA, IPI)
Cellular Component
Manually curated	actin cortical patch (IDA) cytoplasm (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Classical genetics
overexpression	endocytosis: absent
Large-scale survey
null	competitive fitness: decreased Fus-Mid-GFP distribution: abnormal resistance to ethylenediaminetetraacetic acid: decreased resistance to methyl methanesulfonate: decreased resistance to acrolein: decreased viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	47 total interaction(s) for 30 unique genes/features.
Physical Interactions	Affinity Capture-MS: 10 Affinity Capture-RNA: 2 Affinity Capture-Western: 4 Biochemical Activity: 1 Co-localization: 1 Protein-peptide: 3 Reconstituted Complex: 1 Two-hybrid: 17
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 4 Phenotypic Enhancement: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:762428 to 763153 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..726 762428..763153 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB382 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003368