PRE9/YGR135W Summary

PRE9 BASIC INFORMATION

Standard Name	PRE9
Systematic Name	YGR135W
Feature Type	ORF, Verified
Description	Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform (1, 2)
Name Description	PRoteinase yscE

GO Annotations	All PRE9 GO evidence and references
	View Computational GO annotations for PRE9
Molecular Function
Manually curated	endopeptidase activity (TAS)
Biological Process
Manually curated	filamentous growth (IMP) ubiquitin-dependent protein catabolic process (TAS)
Cellular Component
Manually curated	proteasome core complex, alpha-subunit complex (IPI) proteasome storage granule (IDA)

Mutant Phenotype	All PRE9 Phenotype details and references
Classical genetics
null	lipids accumulation: increased prion formation: decreased resistance to 4-nitroquinoline N-oxide: increased resistance to methyl methanesulfonate: increased resistance to camptothecin: increased resistance to canavanine: decreased resistance to L-azetidine-2-carboxylic acid: decreased resistance to bortezomib: decreased toxin resistance: decreased
Large-scale survey
null	chitin deposition: normal competitive fitness: decreased heat sensitivity: increased Protein A-nonstop protein accumulation: increased resistance to Calcofluor White: normal resistance to oleate: decreased resistance to acetaldehyde: decreased resistance to propan-1-ol: decreased sporulation: decreased viable

Interactions	PRE9 All interactions details and references
	304 total interaction(s) for 185 unique genes/features.
Physical Interactions	Affinity Capture-MS: 59 Affinity Capture-RNA: 1 Affinity Capture-Western: 9 Biochemical Activity: 6 Co-fractionation: 4 Co-purification: 1 PCA: 2 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 2 Phenotypic Enhancement: 83 Phenotypic Suppression: 47 Synthetic Growth Defect: 36 Synthetic Lethality: 32 Synthetic Rescue: 17

Sequence Information

ChrVII:761397 to 762173 | |

Last Update

Coordinates: 2004-07-20 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..777	761397..762173	2004-07-20	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003367

PRE9 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PRE9

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PRE9]

1)	Hochstrasser M (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39
2)	Kusmierczyk AR, et al. (2008) A multimeric assembly factor controls the formation of alternative 20S proteasomes. Nat Struct Mol Biol 15(3):237-44

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