PEX4/YGR133W Summary Help

Standard Name PEX4 1
Systematic Name YGR133W
Alias PAS2 2 , UBC10 3
Feature Type ORF, Verified
Description Peroxisomal ubiquitin conjugating enzyme; required for peroxisomal matrix protein import and peroxisome biogenesis (2, 3, 4, 5)
Name Description PEroXin 1
Chromosomal Location
ChrVII:756896 to 757447 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PEX4 GO evidence and references
  View Computational GO annotations for PEX4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
102 total interaction(s) for 72 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 4
  • Co-crystal Structure: 2
  • PCA: 5
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 59
  • Phenotypic Enhancement: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 183
Molecular Weight (Da) 21,118
Isoelectric Point (pI) 5.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:756896 to 757447 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..552 756896..757447 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003365
References cited on this page View Complete Literature Guide for PEX4
1) Distel B, et al.  (1996) A unified nomenclature for peroxisome biogenesis factors. J Cell Biol 135(1):1-3
2) Erdmann R, et al.  (1989) Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 86(14):5419-23
3) Wiebel FF and Kunau WH  (1992) The Pas2 protein essential for peroxisome biogenesis is related to ubiquitin-conjugating enzymes. Nature 359(6390):73-6
4) Eckert JH and Johnsson N  (2003) Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci 116(Pt 17):3623-34
5) Kragt A, et al.  (2005) The Saccharomyces cerevisiae peroxisomal import receptor Pex5p is monoubiquitinated in wild type cells. J Biol Chem 280(9):7867-74