NUP57/YGR119C Summary Help

NUP57 BASIC INFORMATION

Standard Name NUP57
Systematic Name YGR119C
Feature Type ORF, Verified
Description Nucleoporin, essential subunit of the nuclear pore complex (NPC), functions as the organizing center of an NPC subcomplex containing Nsp1p, Nup49p, Nup57p, and Nic96p (1, 2 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP57 GO evidence and references
    View Computational GO annotations for NUP57
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All NUP57 Phenotype details and references
Classical genetics
reduction of function
Large-scale survey
conditional
null
repressible
Interactions NUP57 All interactions details and references
148 total interaction(s) for 96 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 36
  • Affinity Capture-Western: 7
  • Co-purification: 2
  • PCA: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 19
Genetic Interactions
  • Phenotypic Enhancement: 42
  • Phenotypic Suppression: 30
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
Sequence Information
ChrVII:729676 to 728051 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1626 729676..728051 2004-07-20 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003351

NUP57 RESOURCES

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Expression Summary histogram

ADDITIONAL INFORMATION for NUP57

SUMMARY PARAGRAPH for NUP57

NUP57 encodes an essential nuclear pore protein (2). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 3, 4, 5, 6). The structure of the vertebrate NPC has been studied extensively; recent reviews include 7, 8, 9, and 10. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (11, 12). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 3, 4, 13, 14, 15). Mutations in NUP57 cause defects in nuclear protein import and nuclear RNA export (3), and can alter NPC assembly (16); genetic interactions have been detected between NUP57 and genes encoding other nucleoporins (2, 3). Nup57p is one of a group of nucleoporins that contain multiple repeats of the amino acids GLFG (2, 3); however, the repeats in Nup57p are not essential for NPC function (17). Nup57p and Nup49p are very similar, and may have arisen by gene duplication (3). Similar nucleoporins are also found in rat and S. pombe (3). Nup57p is found in a subcomplex of the NPC, often called the Nsp1p subcomplex, that also contains Nsp1p, Nup49p, and Nic96p (3, 18, 19, 2). This complex has been detected at both the nuclear and cytoplasmic periphery of the NPC central channel (1). Further, Nup49, Nup57p, and the C-terminus of Nsp1p have been expressed in E. coli, and form a complex in vitro (18).

Last updated: 1999-07-30

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP57]

1) Fahrenkrog B, et al.  (1998) Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol 143(3):577-88
2) Grandi P, et al.  (1995) Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. EMBO J 14(1):76-87
3) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
4) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
6) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
7) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
8) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
9) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
10) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
11) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
12) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
13) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
14) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
15) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
16) Bucci M and Wente SR  (1998) A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly. Mol Biol Cell 9(9):2439-61
17) Iovine MK, et al.  (1995) The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor. J Cell Biol 131(6 Pt 2):1699-713
18) Schlaich NL, et al.  (1997) In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p. Mol Biol Cell 8(1):33-46
19) Grandi P, et al.  (1993) Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J 12(8):3061-71