SPT6/YGR116W Summary

SPT6/YGR116W Summary

Standard Name	SPT6 1
Systematic Name	YGR116W
Alias	CRE2 2 , SSN20 3
Feature Type	ORF, Verified
Description	Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region (4, 5, 6, 7)
Name Description	SuPpressor of Ty's 1

Chromosomal Location	ChrVII:720409 to 724764 \| ORF Map \| GBrowse

	Genetic position: 87.64 cM

Gene Ontology Annotations All SPT6 GO evidence and references

	View Computational GO annotations for SPT6
Molecular Function
Manually curated	core RNA polymerase II binding transcription factor activity (IMP, IPI) DNA binding transcription antitermination factor activity (IDA, IGI, IMP) histone binding (IDA) nucleosome binding (IDA, IMP)
Biological Process
Manually curated	chromatin maintenance (IMP) chromatin remodeling (IGI, IMP) negative regulation of transcription from RNA polymerase II promoter (IMP) negative regulation of transcription from RNA polymerase II promoter by glucose (IMP) nucleosome assembly (IDA) nucleosome organization (IMP) poly(A)+ mRNA export from nucleus (IMP) positive regulation of transcription elongation from RNA polymerase II promoter (IDA) positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription involved in G1/S phase of mitotic cell cycle (IMP) regulation of histone H3-K36 methylation (IDA, IMP) regulation of mRNA 3'-end processing (IGI, IMP) regulation of nucleosome density (IMP) regulation of transcription by chromatin organization (IGI, IMP) regulation of transcription from RNA polymerase II promoter in response to stress (IMP) regulation of transcriptional start site selection at RNA polymerase II promoter (IMP) transcription antitermination (IGI, IMP)
Cellular Component
Manually curated	nucleus (IDA) transcriptionally active chromatin (IDA)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All SPT6 Phenotype evidence and references

Classical genetics
conditional	heat sensitivity: increased
null	inviable
unspecified	chromosome segregation: decreased heat sensitivity: increased resistance to 6-azauracil: decreased
Large-scale survey
null	filamentous growth: decreased haploinsufficient heat sensitivity: increased invasive growth: absent inviable toxin resistance: decreased
overexpression	vegetative growth: decreased
reduction of function	competitive fitness: increased
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SPT6 Interaction evidence and references

	107 total interaction(s) for 68 unique genes/features.
Physical Interactions	Affinity Capture-MS: 33 Affinity Capture-RNA: 2 Affinity Capture-Western: 4 Biochemical Activity: 1 Co-crystal Structure: 1 Co-fractionation: 1 Co-localization: 1 Protein-peptide: 1 Reconstituted Complex: 2 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 3 Phenotypic Suppression: 3 Positive Genetic: 11 Synthetic Growth Defect: 17 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 10 Synthetic Rescue: 10
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SPT6 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:720409 to 724764 | |

SGD ORF map

: 87.64 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4356	720409..724764	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003348

References cited on this page View Complete Literature Guide for SPT6

1)	Winston F, et al. (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2)	Denis CL (1984) Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II. Genetics 108(4):833-44
3)	Neigeborn L, et al. (1986) Suppressors of SNF2 mutations restore invertase derepression and cause temperature-sensitive lethality in yeast. Genetics 112(4):741-53
4)	Bortvin A and Winston F (1996) Evidence that Spt6p controls chromatin structure by a direct interaction with histones. Science 272(5267):1473-6
5)	Hartzog GA, et al. (1998) Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev 12(3):357-69
6)	Kaplan CD, et al. (2003) Transcription elongation factors repress transcription initiation from cryptic sites. Science 301(5636):1096-9
7)	Close D, et al. (2011) Crystal Structures of the S. cerevisiae Spt6 Core and C-Terminal Tandem SH2 Domain. J Mol Biol 408(4):697-713