VMA21/YGR105W Summary Help

Standard Name VMA21
Systematic Name YGR105W
Feature Type ORF, Verified
Description Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) (1, 2, 3, 4, 5)
Chromosomal Location
ChrVII:698599 to 698832 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All VMA21 GO evidence and references
  View Computational GO annotations for VMA21
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
661 total interaction(s) for 583 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • PCA: 11

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 84
  • Phenotypic Enhancement: 3
  • Positive Genetic: 30
  • Synthetic Growth Defect: 504
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 77
Molecular Weight (Da) 8,435
Isoelectric Point (pI) 4.91
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:698599 to 698832 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..234 698599..698832 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003337
References cited on this page View Complete Literature Guide for VMA21
1) Hill KJ and Stevens TH  (1994) Vma21p is a yeast membrane protein retained in the endoplasmic reticulum by a di-lysine motif and is required for the assembly of the vacuolar H(+)-ATPase complex. Mol Biol Cell 5(9):1039-50
2) Hirata R, et al.  (1993) VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae. J Biol Chem 268(2):961-7
3) Graham LA and Stevens TH  (1999) Assembly of the yeast vacuolar proton-translocating ATPase. J Bioenerg Biomembr 31(1):39-47
4) Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808
5) Ramachandran N, et al.  (2013) VMA21 deficiency prevents vacuolar ATPase assembly and causes autophagic vacuolar myopathy. Acta Neuropathol 125(3):439-57