CTT1/YGR088W Summary Help

Standard Name CTT1 1
Systematic Name YGR088W
Alias SPS101 2
Feature Type ORF, Verified
Description Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide (3 and see Summary Paragraph)
Name Description CaTalase T 1
Chromosomal Location
ChrVII:654634 to 656322 | ORF Map | GBrowse
Gene Ontology Annotations All CTT1 GO evidence and references
  View Computational GO annotations for CTT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 14 genes
Classical genetics
Large-scale survey
37 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Co-localization: 2
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 15
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 562
Molecular Weight (Da) 64,583
Isoelectric Point (pI) 6.51
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:654634 to 656322 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2006-01-23
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1689 654634..656322 2011-02-03 2006-01-23
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003320

CTT1 encodes cytosolic catalase T, which is involved in hydrogen peroxide detoxification (4, 5). Catalases break down hydrogen peroxide into dioxygen (O2) and water molecules (reviewed in 6). Ctt1p activity is important during the oxidative stress response and in protecting proteins against oxidative inactivation (7, 8, and reviewed in 6). Catalase activity is also increased during caloric restriction and during oxidative stress caused by the processes of aging, acid stress adaptation, and thermotolerance (9, 10, 11, 12).

CTT1 expression is induced under various stresses, such as carbon starvation and oxidative, osmotic, or freeze-thaw stress (reviewed in 13). This transcriptional response is mediated by transcription factors such as Msn2p/Msn4p, Hog1p, Hap1p, Yap1p, and Zap1p acting upon upstream activating elements (UAS) in the CTT1 promoter, including the stress response element (STRE) and a heme control region (14, 15, 16, 17, 9, 18). The localization of Ctt1p is regulated during cytokinesis, with a higher proportion of active Ctt1p found in daughter cells (19).

The S. cerevisiae genome encodes a second catalase, peroxisomal catalase A (encoded by CTA1), and although these two enzymes are functionally similar, Cta1p is more similar in sequence to the peroxiosomal catalases from the cow Bos taurus and the pathogenic yeast Candida tropicalis than it is to Ctt1p (20). All yeast species appear to have a Cta1p ortholog however not all yeasts have an ortholog of Ctt1p (21).

Last updated: 2010-02-10 Contact SGD

References cited on this page View Complete Literature Guide for CTT1
1) Traczyk A, et al.  (1985) Catalase T deficient mutants of Saccharomyces cerevisiae. Acta Microbiol Pol 34(3-4):231-41
2) Neiman A  (2007) personal communication
3) Grant CM, et al.  (1998) Glutathione and catalase provide overlapping defenses for protection against hydrogen peroxide in the yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun 253(3):893-8
4) Cohen G, et al.  (1985) Isolation of the catalase A gene of Saccharomyces cerevisiae by complementation of the cta1 mutation. Mol Gen Genet 200(1):74-9
5) Petrova VY, et al.  (2004) Dual targeting of yeast catalase A to peroxisomes and mitochondria. Biochem J 380(Pt 2):393-400
6) Jamieson DJ  (1998) Oxidative stress responses of the yeast Saccharomyces cerevisiae. Yeast 14(16):1511-27
7) Wieser R, et al.  (1991) Heat shock factor-independent heat control of transcription of the CTT1 gene encoding the cytosolic catalase T of Saccharomyces cerevisiae. J Biol Chem 266(19):12406-11
8) Lushchak VI and Gospodaryov DV  (2005) Catalases protect cellular proteins from oxidative modification in Saccharomyces cerevisiae. Cell Biol Int 29(3):187-92
9) Marchler G, et al.  (1993) A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J 12(5):1997-2003
10) Agarwal S, et al.  (2005) Caloric restriction augments ROS defense in S. cerevisiae, by a Sir2p independent mechanism. Free Radic Res 39(1):55-62
11) Giannattasio S, et al.  (2005) Acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-induced programmed cell death. Gene 354():93-8
12) Davidson JF, et al.  (1996) Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 93(10):5116-21
13) Herrero E, et al.  (2008) Redox control and oxidative stress in yeast cells. Biochim Biophys Acta 1780(11):1217-35
14) Martinez-Pastor MT, et al.  (1996) The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). EMBO J 15(9):2227-35
15) Schuller C, et al.  (1994) The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTT1 gene. EMBO J 13(18):4382-9
16) Lee J, et al.  (1999) Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. J Biol Chem 274(23):16040-6
17) Wu CY, et al.  (2008) Differential control of Zap1-regulated genes in response to zinc deficiency in Saccharomyces cerevisiae. BMC Genomics 9:370
18) Winkler H, et al.  (1988) Co-ordinate control of synthesis of mitochondrial and non-mitochondrial hemoproteins: a binding site for the HAP1 (CYP1) protein in the UAS region of the yeast catalase T gene (CTT1). EMBO J 7(6):1799-804
19) Erjavec N and Nystrom T  (2007) Sir2p-dependent protein segregation gives rise to a superior reactive oxygen species management in the progeny of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 104(26):10877-81
20) Cohen G, et al.  (1988) Sequence of the Saccharomyces cerevisiae CTA1 gene and amino acid sequence of catalase A derived from it. Eur J Biochem 176(1):159-63
21) Aksam EB, et al.  (2009) Preserving organelle vitality: peroxisomal quality control mechanisms in yeast. FEMS Yeast Res 9(6):808-20