PIL1/YGR086C Summary Help

Standard Name PIL1 1, 2
Systematic Name YGR086C
Feature Type ORF, Verified
Description Eisosome core component; eisosomes are large immobile cell cortex structures associated with endocytosis; detected in phosphorylated state in mitochondria; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; member of BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress (2, 3, 4, 5, 6, 7)
Name Description Phosphorylation Inhibited by Long chain bases 2
Chromosomal Location
ChrVII:650617 to 649598 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PIL1 GO evidence and references
  View Computational GO annotations for PIL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 13 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
overexpression
Resources
287 total interaction(s) for 182 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 106
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 1
  • PCA: 7
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 3
  • Negative Genetic: 111
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 2
  • Positive Genetic: 24
  • Synthetic Growth Defect: 3
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 339
Molecular Weight (Da) 38,349
Isoelectric Point (pI) 4.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:650617 to 649598 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1020 650617..649598 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003318
References cited on this page View Complete Literature Guide for PIL1
1) Samanta MP and Liang S  (2003) Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci U S A 100(22):12579-83
2) Zhang X, et al.  (2004) Pil1p and Lsp1p negatively regulate the 3-phosphoinositide-dependent protein kinase-like kinase Pkh1p and downstream signaling pathways Pkc1p and Ypk1p. J Biol Chem 279(21):22030-8
3) Walther TC, et al.  (2006) Eisosomes mark static sites of endocytosis. Nature 439(7079):998-1003
4) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
5) Ziolkowska NE, et al.  (2011) Eisosome-driven plasma membrane organization is mediated by BAR domains.LID - 10.1038/nsmb.2080 [doi] Nat Struct Mol Biol ()
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Mascaraque V, et al.  (2013) Phosphoproteomic analysis of protein kinase C signaling in Saccharomyces cerevisiae reveals Slt2 mitogen-activated protein kinase (MAPK)-dependent phosphorylation of eisosome core components. Mol Cell Proteomics 12(3):557-74