TWF1/YGR080W Summary Help

Standard Name TWF1 1
Systematic Name YGR080W
Feature Type ORF, Verified
Description Twinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly (1, 2, 3)
Name Description TWinFilin 1
Gene Product Alias twinfilin 1
Chromosomal Location
ChrVII:642010 to 643008 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TWF1 GO evidence and references
  View Computational GO annotations for TWF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
43 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Co-fractionation: 1
  • Co-localization: 1
  • PCA: 2
  • Reconstituted Complex: 5

Genetic Interactions
  • Negative Genetic: 16
  • Positive Genetic: 4
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 332
Molecular Weight (Da) 37,070
Isoelectric Point (pI) 5.56
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:642010 to 643008 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..999 642010..643008 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003312
References cited on this page View Complete Literature Guide for TWF1
1) Goode BL, et al.  (1998) Regulation of the cortical actin cytoskeleton in budding yeast by twinfilin, a ubiquitous actin monomer-sequestering protein. J Cell Biol 142(3):723-33
2) Palmgren S, et al.  (2001) Interactions with PIP2, ADP-actin monomers, and capping protein regulate the activity and localization of yeast twinfilin. J Cell Biol 155(2):251-60
3) Moseley JB, et al.  (2006) Twinfilin is an actin-filament-severing protein and promotes rapid turnover of actin structures in vivo. J Cell Sci 119(Pt 8):1547-57