PRP38/YGR075C Summary Help

Standard Name PRP38 1
Systematic Name YGR075C
Feature Type ORF, Verified
Description Unique component of the U4/U6.U5 tri-snRNP particle; tri-snRNP is required for conformational changes which result in the catalytic activation of the spliceosome; dispensable for spliceosome assembly (2, 3)
Name Description Pre-mRNA Processing
Chromosomal Location
ChrVII:636875 to 636147 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PRP38 GO evidence and references
  View Computational GO annotations for PRP38
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
null
overexpression
reduction of function
unspecified
Resources
61 total interaction(s) for 38 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Co-purification: 4
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 9
  • Dosage Rescue: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 6
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 242
Molecular Weight (Da) 27,966
Isoelectric Point (pI) 5.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:636875 to 636147 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..729 636875..636147 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003307
References cited on this page View Complete Literature Guide for PRP38
1) Blanton S, et al.  (1992) PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels. Mol Cell Biol 12(9):3939-47
2) Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31
3) Xie J, et al.  (1998) Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation. EMBO J 17(10):2938-46