Standard Name	PEF1 1
Systematic Name	YGR058W
Feature Type	ORF, Verified
Description	Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly (1, 2)
Name Description	Penta-EF-Hand protein 1

Chromosomal Location	ChrVII:606136 to 607143 | ORF Map | GBrowse

	View Computational GO annotations for PEF1
Molecular Function
Manually curated	calcium ion binding (IDA) zinc ion binding (IDA)
Biological Process
Manually curated	axial cellular bud site selection (IMP) bipolar cellular bud site selection (IMP) regulation of COPII vesicle coating (IDA, IMP, IPI)
Cellular Component
Manually curated	cellular bud neck (IDA) cytosol (IDA) site of polarized growth (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Classical genetics
null	axial budding pattern: abnormal bipolar budding pattern: decreased rate resistance to perfluorononanoic acid: decreased resistance to sodium dodecyl sulfate: decreased resistance to ethylene glycol bis(2-aminoethyl)tetraacetic acid: decreased viable
overexpression	vegetative growth: decreased
Large-scale survey
null	competitive fitness: decreased endocytosis: decreased haploinsufficient resistance to tunicamycin: decreased resistance to PM02734: decreased resistance to wortmannin: decreased resistance to sirolimus: decreased resistance to fluconazole: increased resistance to fenpropimorph: increased resistance to bleomycin: increased resistance to camptothecin: decreased resistance to hygromycin B: decreased resistance to 1,4-dithiothreitol: decreased sporulation: normal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	101 total interaction(s) for 82 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 2 Biochemical Activity: 1 Reconstituted Complex: 1 Two-hybrid: 10
Genetic Interactions	Negative Genetic: 42 Phenotypic Enhancement: 6 Phenotypic Suppression: 25 Positive Genetic: 13
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:606136 to 607143 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1008 606136..607143 2011-02-03 2011-02-03
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003290