CAX4/YGR036C Summary Help

Standard Name CAX4 1
Systematic Name YGR036C
Alias CWH8 2
Feature Type ORF, Verified
Description Dolichyl pyrophosphate (Dol-P-P) phosphatase; has a luminally oriented active site in the ER; cleaves the anhydride linkage in Dol-P-P; required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation (3 and see Summary Paragraph)
Name Description CAlmodulin-dependent in cmd one two twenty-siX 1
Chromosomal Location
ChrVII:558868 to 558149 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CAX4 GO evidence and references
  View Computational GO annotations for CAX4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 63 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
51 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • PCA: 1

Genetic Interactions
  • Negative Genetic: 21
  • Positive Genetic: 25
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 239
Molecular Weight (Da) 27,649
Isoelectric Point (pI) 8.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:558868 to 558149 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..720 558868..558149 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003268
SUMMARY PARAGRAPH for CAX4

During N-linked glycosylation of proteins, oligosaccharide chains are assembled on the carrier molecule dolichyl pyrophosphate in the following order: 2 molecules of N-acetylglucosamine (GlcNAc), 9 molecules of mannose, and 3 molecules of glucose. These 14-residue oligosaccharide cores are then transferred to asparagine residues on nascent polypeptide chains in the endoplasmic reticulum (ER). As proteins progress through the Golgi apparatus, the oligosaccharide cores are modified by trimming and extension to generate a diverse array of glycosylated proteins (reviewed in 4, 5).

Cax4p converts dolichyl pyrophosphate (Dol-P-P) to dolichyl phosphate (Dol-P) in the lumen of the ER (3), possibly to recycle Dol-P-P (6); Dol-P-P is the byproduct of the transfer of a LLO to a protein, and Dol-P is the substrate on the cytosolic side of the ER on which many of the early reactions in N-linked glycosylation occur (see ALG7, DPM1, and ALG5).

Cells lacking Cax4p grow slowly, exhibit decreased levels of N-linked glycosylation--although those LLO's that are synthesized are full-length (2)--and accumulate Dol-P-P (3). They have an anomalous cell wall and are sensitive to Calcofluor white (2, 7), hygromycin B (2), and FK506 (1) and resistant to vanadate (2). Overexpression of RER2, SEC59, or LPP1--which generate Dol-P through other pathways--partially suppresses the cax4 phenotype (3). Mutation of CAX4 causes a synthetic lethal interaction with calmodulin (cmd1) and exhibits defective actin organization (1). The mouse CAX4 homolog DOLPP1 complements deletion of CAX4 (8).

Last updated: 2005-07-07 Contact SGD

References cited on this page View Complete Literature Guide for CAX4
1) Sekiya-Kawasaki M, et al.  (1998) Identification of functional connections between calmodulin and the yeast actin cytoskeleton. Genetics 150(1):43-58
2) van Berkel MA, et al.  (1999) The Saccharomyces cerevisiae CWH8 gene is required for full levels of dolichol-linked oligosaccharides in the endoplasmic reticulum and for efficient N-glycosylation. Glycobiology 9(3):243-53
3) Fernandez F, et al.  (2001) The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae. J Biol Chem 276(44):41455-64
4) Herscovics A and Orlean P  (1993) Glycoprotein biosynthesis in yeast. FASEB J 7(6):540-50
5) Burda P and Aebi M  (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426(2):239-57
6) Schenk B, et al.  (2001) The ins(ide) and out(side) of dolichyl phosphate biosynthesis and recycling in the endoplasmic reticulum. Glycobiology 11(5):61R-70R
7) Ram AF, et al.  (1994) A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white. Yeast 10(8):1019-30
8) Rush JS, et al.  (2002) Identification and characterization of a cDNA encoding a dolichyl pyrophosphate phosphatase located in the endoplasmic reticulum of mammalian cells. J Biol Chem 277(47):45226-34