RAI1/YGL246C Summary Help

Standard Name RAI1 1
Systematic Name YGL246C
Feature Type ORF, Verified
Description Nuclear protein with decapping endonuclease activity; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z (1, 2, 3, 4)
Name Description Rat1p Interacting Protein 1
Chromosomal Location
ChrVII:38780 to 37617 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RAI1 GO evidence and references
  View Computational GO annotations for RAI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
repressible
Resources
84 total interaction(s) for 64 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 55
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Biochemical Activity: 3

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 7
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 387
Molecular Weight (Da) 44,509
Isoelectric Point (pI) 6.52
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:38780 to 37617 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1164 38780..37617 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003215
References cited on this page View Complete Literature Guide for RAI1
1) Xue Y, et al.  (2000) Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Mol Cell Biol 20(11):4006-15
2) Sydorskyy Y, et al.  (2003) Intersection of the Kap123p-mediated nuclear import and ribosome export pathways. Mol Cell Biol 23(6):2042-54
3) Jiao X, et al.  (2010) Identification of a quality-control mechanism for mRNA 5'-end capping. Nature 467(7315):608-11
4) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30