RTF1/YGL244W Summary Help

Standard Name RTF1 1
Systematic Name YGL244W
Alias CSL3 2
Feature Type ORF, Verified
Description Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay (1, 3, 4, 5, 6)
Name Description Restores TBP Function 1
Chromosomal Location
ChrVII:41498 to 43174 | ORF Map | GBrowse
Gene Ontology Annotations All RTF1 GO evidence and references
  View Computational GO annotations for RTF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 8 genes
Regulators 4 genes
Classical genetics
Large-scale survey
585 total interaction(s) for 325 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 81
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 21
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 1
  • Protein-RNA: 4
  • Reconstituted Complex: 3
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 6
  • Negative Genetic: 263
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 63
  • Synthetic Growth Defect: 57
  • Synthetic Lethality: 58
  • Synthetic Rescue: 14

Expression Summary
Length (a.a.) 558
Molecular Weight (Da) 65,868
Isoelectric Point (pI) 5.12
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:41498 to 43174 | ORF Map | GBrowse
Last Update Coordinates: 1999-07-17 | Sequence: 1999-07-17
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1677 41498..43174 1999-07-17 1999-07-17
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003213
References cited on this page View Complete Literature Guide for RTF1
1) Stolinski LA, et al.  (1997) Identification of RTF1, a novel gene important for TATA site selection by TATA box-binding protein in Saccharomyces cerevisiae. Mol Cell Biol 17(8):4490-500
2) Baker RE, et al.  (1998) Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics 149(1):73-85
3) Mueller CL and Jaehning JA  (2002) Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mol Cell Biol 22(7):1971-80
4) Krogan NJ, et al.  (2002) RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Mol Cell Biol 22(20):6979-92
5) Warner MH, et al.  (2007) Rtf1 is a multifunctional component of the paf1 complex that regulates gene expression by directing cotranscriptional histone modification. Mol Cell Biol 27(17):6103-15
6) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64