DOC1/YGL240W Summary

DOC1 BASIC INFORMATION

Standard Name	DOC1
Systematic Name	YGL240W
Alias	APC10
Feature Type	ORF, Verified
Description	Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis (1, 2, 3, 4, 5)
Name Description	Destruction Of Cyclin B 1

GO Annotations	All DOC1 GO evidence and references
	View Computational GO annotations for DOC1
Molecular Function
Manually curated	enzyme regulator activity (IDA)
Biological Process
Manually curated	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA, IMP, IPI) cyclin catabolic process (TAS) mitotic metaphase/anaphase transition (TAS) mitotic sister chromatid segregation (TAS) mitotic spindle elongation (TAS)
Cellular Component
Manually curated	anaphase-promoting complex (IDA)
High-throughput	mitochondrion (IDA)

Mutant Phenotype	All DOC1 Phenotype details and references
Classical genetics
conditional	cell cycle progression through the G2/M phase transition: arrested Clb2p accumulation: increased
null	cell cycle passage through the metaphase-anaphase transition: delayed colony sectoring: increased mitochondrial genome maintenance: abnormal vegetative growth: decreased rate
Large-scale survey
null	acid pH resistance: decreased glycogen accumulation: decreased chitin deposition: increased competitive fitness: decreased resistance to Calcofluor White: decreased resistance to L-1,4-dithiothreitol: decreased resistance to hydroxyurea: decreased resistance to cisplatin: decreased resistance to doxorubicin: decreased respiratory growth: decreased rate respiratory growth: absent toxin resistance: decreased utilization of carbon source: decreased viable

Interactions	DOC1 All interactions details and references
	104 total interaction(s) for 53 unique genes/features.
Physical Interactions	Affinity Capture-MS: 41 Affinity Capture-Western: 5 Biochemical Activity: 1 Co-fractionation: 1 PCA: 1 Two-hybrid: 12
Genetic Interactions	Dosage Lethality: 5 Dosage Rescue: 2 Phenotypic Enhancement: 1 Synthetic Growth Defect: 6 Synthetic Lethality: 26 Synthetic Rescue: 3

Sequence Information

ChrVII:48613 to 49365 | |

Last Update

Coordinates: 2003-09-22 | Sequence: 2003-09-22

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..753	48613..49365	2003-09-22	2003-09-22

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003209

DOC1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for DOC1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for DOC1]

1)	Hwang LH and Murray AW (1997) A novel yeast screen for mitotic arrest mutants identifies DOC1, a new gene involved in cyclin proteolysis. Mol Biol Cell 8(10):1877-87
2)	Grossberger R, et al. (1999) Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. J Biol Chem 274(20):14500-7
3)	Au SW, et al. (2002) Implications for the ubiquitination reaction of the anaphase-promoting complex from the crystal structure of the Doc1/Apc10 subunit. J Mol Biol 316(4):955-68
4)	Carroll CW and Morgan DO (2002) The Doc1 subunit is a processivity factor for the anaphase-promoting complex. Nat Cell Biol 4(11):880-7
5)	Passmore LA, et al. (2003) Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J 22(4):786-96

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