VAM7/YGL212W Summary Help

Standard Name VAM7 1
Systematic Name YGL212W
Alias VPS43 2 , VPL24 2
Feature Type ORF, Verified
Description Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress (1, 3, 4, 5)
Name Description VAcuolar Morphogenesis
Chromosomal Location
ChrVII:91432 to 92382 | ORF Map | GBrowse
Gene Ontology Annotations All VAM7 GO evidence and references
  View Computational GO annotations for VAM7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
441 total interaction(s) for 258 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 43
  • Co-fractionation: 3
  • Co-purification: 9
  • FRET: 1
  • PCA: 5
  • Reconstituted Complex: 28
  • Two-hybrid: 48

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 234
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 27
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 8
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 316
Molecular Weight (Da) 36,711
Isoelectric Point (pI) 9
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:91432 to 92382 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..951 91432..92382 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003180
References cited on this page View Complete Literature Guide for VAM7
1) Sato TK, et al.  (1998) Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking. Mol Cell Biol 18(9):5308-19
2) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
3) Ungermann C and Wickner W  (1998) Vam7p, a vacuolar SNAP-25 homolog, is required for SNARE complex integrity and vacuole docking and fusion. EMBO J 17(12):3269-76
4) Yu JW and Lemmon MA  (2001) All phox homology (PX) domains from Saccharomyces cerevisiae specifically recognize phosphatidylinositol 3-phosphate. J Biol Chem 276(47):44179-84
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76