Standard Name	VAM7 1
Systematic Name	YGL212W
Alias	VPS43 2 , VPL24 2
Feature Type	ORF, Verified
Description	Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress (1, 3, 4, 5)
Name Description	VAcuolar Morphogenesis

Chromosomal Location	ChrVII:91432 to 92382 | ORF Map | GBrowse

	View Computational GO annotations for VAM7
Molecular Function
Manually curated	phosphatidylinositol-3-phosphate binding (IDA) SNAP receptor activity (IDA, ISS)
Biological Process
Manually curated	piecemeal microautophagy of nucleus (IMP) vacuole fusion, non-autophagic (IDA, IMP) vesicle fusion (IDA)
Cellular Component
Manually curated	fungal-type vacuole membrane (IDA, IMP) SNARE complex (IMP, IPI)

Classical genetics
null	autophagy: absent metal resistance: decreased GFP-Osh1p accumulation: increased carboxypeptidase Y (Prc1p) modification: decreased replicative lifespan: decreased resistance to Calcofluor White: decreased vacuolar morphology: abnormal viable
Large-scale survey
null	rubidium(1+) accumulation: decreased arginine accumulation: decreased glutathione excretion: increased chitin deposition: normal competitive fitness: decreased dessication resistance: decreased haploinsufficient ionic stress resistance: decreased metal resistance: decreased mitophagy: absent Pho8p (ALP) modification: decreased Cps1p (CPS) modification: decreased resistance to monensin: decreased resistance to Calcofluor White: decreased resistance to sirolimus: decreased resistance to acetic acid: increased resistance to cycloheximide: decreased resistance to hygromycin B: decreased resistance to St. John's Wort extract: decreased resistance to PM02734: decreased resistance to ethanol: decreased resistance to caffeine: decreased resistance to fluconazole: decreased resistance to tunicamycin: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to neothyonidioside: decreased resistance to spermine: decreased resistance to dimethyl sulfoxide: decreased resistance to mefloquine: decreased resistance to ciclopirox olamine: decreased resistance to clioquinol: decreased resistance to wortmannin: normal resistance to BPS: decreased resistance to calcium dichloride: decreased sporulation: absent sporulation efficiency: decreased stress resistance: decreased telomere length: decreased transposable element transposition: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	392 total interaction(s) for 231 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 1 Affinity Capture-Western: 40 Co-fractionation: 3 Co-purification: 9 FRET: 1 PCA: 5 Reconstituted Complex: 28 Two-hybrid: 48
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 3 Negative Genetic: 198 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Positive Genetic: 19 Synthetic Growth Defect: 16 Synthetic Lethality: 8 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:91432 to 92382 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..951 91432..92382 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003180