Standard Name	NCS6
Systematic Name	YGL211W
Alias	YGL210W-A , TUC1 1
Feature Type	ORF, Verified
Description	Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae (1, 2, 3)
Name Description	Needs Cla4 to Survive 4

Chromosomal Location	ChrVII:92512 to 93591 | ORF Map | GBrowse

	View Computational GO annotations for NCS6
Molecular Function
Manually curated	tRNA binding (IDA)
Biological Process
Manually curated	protein urmylation (IMP) tRNA wobble position uridine thiolation (IMP) tRNA wobble uridine modification (IMP)
Cellular Component
Manually curated	cytosol (IDA)
High-throughput	mitochondrion (IDA)

Classical genetics
null	tRNA modification: absent tRNA-Glu modification: absent growth in exponential phase: decreased rate resistance to canavanine: decreased
Large-scale survey
null	tRNA modification: abnormal auxotrophy glycogen accumulation: increased competitive fitness: decreased heat sensitivity: increased resistance to quinine: decreased resistance to sirolimus: decreased resistance to hygromycin B: decreased resistance to cycloheximide: decreased resistance to wortmannin: normal resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased sporulation: normal starvation resistance: decreased toxin resistance: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	167 total interaction(s) for 121 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 2 Protein-peptide: 1 Two-hybrid: 3
Genetic Interactions	Negative Genetic: 112 Positive Genetic: 11 Synthetic Growth Defect: 23 Synthetic Lethality: 9
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:92512 to 93591 | ORF Map | GBrowse
	This feature contains embedded feature(s): YGL210W-A
Last Update	Coordinates: 2011-02-03 | Sequence: 2004-07-20
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1080 92512..93591 2011-02-03 2004-07-20
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003179