YPT32/YGL210W Summary

Standard Name	YPT32 1 (see Nomenclature conflict Note)
Systematic Name	YGL210W
Feature Type	ORF, Verified
Description	Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description	Yeast Protein Two 1

Chromosomal Location	ChrVII:93792 to 94460 \| ORF Map \| GBrowse

Gene Ontology Annotations All YPT32 GO evidence and references

	View Computational GO annotations for YPT32
Molecular Function
Manually curated	GTPase activity (IDA, IPI, ISS)
Biological Process
Manually curated	early endosome to Golgi transport (IGI) exocytosis (IMP) vesicle-mediated transport (IGI, IMP)
Cellular Component
Manually curated	endosome (IDA) Golgi apparatus (IDA)
High-throughput	mitochondrial outer membrane (IDA)

Mutant phenotypes All YPT32 Phenotype evidence and references

Classical genetics
null	viable
Large-scale survey
null	competitive fitness: increased resistance to benzo[a]pyrene: decreased resistance to cycloheximide: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All YPT32 Interaction evidence and references

	95 total interaction(s) for 56 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 5 Affinity Capture-Western: 5 Biochemical Activity: 1 Co-purification: 2 PCA: 2 Protein-RNA: 1 Reconstituted Complex: 11 Two-hybrid: 12
Genetic Interactions	Dosage Growth Defect: 3 Dosage Lethality: 2 Dosage Rescue: 14 Negative Genetic: 18 Phenotypic Enhancement: 2 Positive Genetic: 2 Synthetic Growth Defect: 8 Synthetic Lethality: 3 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All YPT32 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:93792 to 94460 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..669	93792..94460	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003178

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
YPT11	Nomenclature conflict	Both YPT32/YGL210W, a GTPase involved in exocytosis and vesicle-mediated transport, and YPT11/YNL304W, a RAB small monomeric GTPase involved in mitochondrial inheritance, have been referred to as YPT11.

SUMMARY PARAGRAPH for YPT32

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 7, 8, 9).

Last updated: 2010-05-18

References cited on this page View Complete Literature Guide for YPT32

1)	Benli M, et al. (1996) Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast. EMBO J 15(23):6460-75
2)	Jedd G, et al. (1997) Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment. J Cell Biol 137(3):563-80
3)	Lazar T, et al. (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
4)	Wang W and Ferro-Novick S (2002) A Ypt32p exchange factor is a putative effector of Ypt1p. Mol Biol Cell 13(9):3336-43
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Cai H, et al. (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
8)	Grosshans BL, et al. (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
9)	Bialek-Wyrzykowska U, et al. (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311