SPT16/YGL207W Summary Help

SPT16 BASIC INFORMATION

Standard Name SPT16
Systematic Name YGL207W
Alias CDC68 1 , SSF1
Feature Type ORF, Verified
Description Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), facilitates RNA Polymerase II transcription elongation through nucleosomes by destabilizing and then reassembling nucleosome structure (2, 3 and see Summary Paragraph)
Name Description SuPpressor of Ty
GO Annotations All SPT16 GO evidence and references
    View Computational GO annotations for SPT16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All SPT16 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
repressible
Interactions SPT16 All interactions details and references
291 total interaction(s) for 134 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 148
  • Affinity Capture-Western: 24
  • Biochemical Activity: 15
  • Co-fractionation: 1
  • PCA: 5
  • Reconstituted Complex: 4
  • Two-hybrid: 2
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 7
  • Dosage Rescue: 9
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 27
  • Synthetic Lethality: 21
  • Synthetic Rescue: 17
Sequence Information
ChrVII:98973 to 102080 | ORF Map | GBrowse
Gbrowse
Genetic position: -134 cM
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3108 98973..102080 2004-07-20 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003175

SPT16 RESOURCES

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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for SPT16

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
SSF1Nomenclature conflictSSF1 has been used in the literature to refer to both SPT16/YGL207W, which encodes a transcription elongation factor and SSF1/YHR066W, which encodes a rRNA binding protein.

SUMMARY PARAGRAPH for SPT16

SPT16 encodes an essential global regulator of transcription (4, 5). When a temperature sensitive spt16 mutant is shifted to the restrictive temperature the abundance of many transcripts is reduced, including some that are required at START, such as CLN1, CLN2, and CLN3 (6). The arrest of spt16 (cdc68) mutants as large unbudded cells is likely due to this negative effect on the transcription of genes needed at START (7, 6, 1). SPT16 was identified as a suppressor of Ty insertion mutations, and because of its phenotypic similarity to some histone mutants, it is thought to play some role in the regulation of chromatin structure (4, 5). Spt16p has been found to physically interact with Pob3p (which has sequence similarity to some HMG chromatin-associated proteins) and the catalytic subunit of DNA polymerase alpha, Pol1p (5, 8). Some of the Spt16p/Pob3p complex in the cell is chromatin associated, and some copurifies with the DNA polymerase alpha-primase complex (5). The N-terminal third of Spt16p is necessary for the maintenance of chromatin repression, but not for activation of genes (9). Homologs of SPT16 have been found in K. lactis and human (10, 9).

NOTE: SSF1 has been used in the literature to refer to both SPT16/YGL207W and SSF1/YHR066W, which encodes a rRNA binding protein.

Last updated: 2000-03-10

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SPT16]

1) Prendergast JA, et al.  (1990) Size selection identifies new genes that regulate Saccharomyces cerevisiae cell proliferation. Genetics 124(1):81-90
2) Formosa T, et al.  (2002) Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure. Genetics 162(4):1557-71
3) Belotserkovskaya R, et al.  (2003) FACT facilitates transcription-dependent nucleosome alteration. Science 301(5636):1090-3
4) Malone EA, et al.  (1991) Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae. Mol Cell Biol 11(11):5710-7
5) Wittmeyer J, et al.  (1999) Spt16 and Pob3 of Saccharomyces cerevisiae form an essential, abundant heterodimer that is nuclear, chromatin-associated, and copurifies with DNA polymerase alpha. Biochemistry 38(28):8961-71
6) Rowley A, et al.  (1991) CDC68, a yeast gene that affects regulation of cell proliferation and transcription, encodes a protein with a highly acidic carboxyl terminus. Mol Cell Biol 11(11):5718-26
7) Wittmeyer J and Formosa T  (1997) The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein. Mol Cell Biol 17(7):4178-90
8) Brewster NK, et al.  (1998) Characterization of the CP complex, an abundant dimer of Cdc68 and Pob3 proteins that regulates yeast transcriptional activation and chromatin repression. J Biol Chem 273(34):21972-9
9) Evans DR, et al.  (1998) The yeast protein complex containing cdc68 and pob3 mediates core-promoter repression through the cdc68 N-terminal domain. Genetics 150(4):1393-405
10) Orphanides G, et al.  (1999) The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins. Nature 400(6741):284-8