COX13/YGL191W Summary Help

Standard Name COX13
Systematic Name YGL191W
Feature Type ORF, Verified
Description Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes (1, 2 and see Summary Paragraph)
Name Description Cytochrome c OXidase
Chromosomal Location
ChrVII:144808 to 145197 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All COX13 GO evidence and references
  View Computational GO annotations for COX13
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Pathways
Classical genetics
null
Resources
29 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 11
  • Co-purification: 1

Genetic Interactions
  • Negative Genetic: 9
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 129
Molecular Weight (Da) 15,021
Isoelectric Point (pI) 8.44
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:144808 to 145197 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..390 144808..145197 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003159
SUMMARY PARAGRAPH for COX13

Cytochrome c oxidase catalyzes the terminal step in the electron transport chain involved in cellular respiration. This multisubunit enzyme of the mitochondrial inner membrane, also known as Complex IV, is composed of three core subunits encoded by the mitochondrial genome (Cox1p, Cox2p, and Cox3p) and eight additional subunits encoded by nuclear genes (Cox4p, Cox5Ap or Cox5Bp, Cox6p, Cox7p, Cox8p, Cox9p, Cox12p, and Cox13p) (3, 4, 5). The phenotype of a mutation affecting any of the genes encoding cytochrome c oxidase subunits, or any of the multiple genes required for expression or assembly of the subunits (reviewed in 6), is a decrease or block in respiratory growth. The inability to respire is not lethal since S. cerevisiae can grow by fermentation, but nonrespiring cells grow more slowly than respiratory-competent cells even on glucose-containing medium, resulting in smaller colony size. Such mutations causing "petite" colonies were first described by Ephrussi and colleagues around 1950 (7).

Cox13p is not required for assembly or activity of cytochrome c oxidase. However, the cox13 null mutation does cause a partial respiratory growth defect (1). Cox13p, also known as subunit VIa, may regulate cytochrome c oxidase activity through its interactions with ATP (1, 8).

Last updated: 2006-08-18 Contact SGD

References cited on this page View Complete Literature Guide for COX13
1) Taanman JW and Capaldi RA  (1993) Subunit VIa of yeast cytochrome c oxidase is not necessary for assembly of the enzyme complex but modulates the enzyme activity. Isolation and characterization of the nuclear-coded gene. J Biol Chem 268(25):18754-61
2) Vukotic M, et al.  (2012) Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab 15(3):336-47
3) Taanman JW and Capaldi RA  (1992) Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme. J Biol Chem 267(31):22481-5
4) Geier BM, et al.  (1995) Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method. Eur J Biochem 227(1-2):296-302
5) Cooper CE, et al.  (1991) Cytochrome c oxidase: structure, function, and membrane topology of the polypeptide subunits. Biochem Cell Biol 69(9):586-607
6) Herrmann JM and Funes S  (2005) Biogenesis of cytochrome oxidase-sophisticated assembly lines in the mitochondrial inner membrane. Gene 354():43-52
7) Ephrussi B and Slonimski PP  (1955) Subcellular units involved in the synthesis of respiratory enzymes in yeast. Nature 176(4495):1207-8
8) Beauvoit B, et al.  (1999) ATP-regulation of cytochrome oxidase in yeast mitochondria: role of subunit VIa. Eur J Biochem 263(1):118-27