NUP49/YGL172W Summary Help

Standard Name NUP49 1
Systematic Name YGL172W
Alias NSP49
Feature Type ORF, Verified
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) (2, 3, 4, 5, 6, 7, 8, 9, 10 and see Summary Paragraph)
Name Description NUclear Pore 11
Chromosomal Location
ChrVII:180700 to 182118 | ORF Map | GBrowse
Gene Ontology Annotations All NUP49 GO evidence and references
  View Computational GO annotations for NUP49
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
123 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 55
  • Affinity Capture-Western: 9
  • Biochemical Activity: 2
  • Co-fractionation: 2
  • Co-localization: 2
  • Co-purification: 3
  • Far Western: 3
  • FRET: 2
  • PCA: 6
  • Reconstituted Complex: 9
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 4
  • Phenotypic Enhancement: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 472
Molecular Weight (Da) 49,142
Isoelectric Point (pI) 6.06
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:180700 to 182118 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1419 180700..182118 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003140

NUP49 encodes an essential nuclear pore protein (1). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 12, 13, 14, 15). The structure of the vertebrate NPC has been studied extensively; recent reviews include 16, 17, 18, and 19. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (20, 21). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 12, 13, 22, 23, 24). Nup49p is one of a group of nucleoporins that contain multiple repeats of the amino acids GLFG (25, 12); however, the Nup49p repeats are not essential for NPC function (26). Nup49p and Nup57p are very similar, and may have arisen by gene duplication (12). Similar nucleoporins are also found in rat and S. pombe (12). Nup49p is found in a subcomplex of the NPC, often called the Nsp1p subcomplex, that also contains Nsp1p, Nup57p, and Nic96p, (12, 27, 2). This complex has been detected at both the nuclear and cytoplasmic periphery of the NPC central channel (28). Further, Nup49, Nup57p, and the C-terminus of Nsp1p have been expressed in E. coli, and form a complex in vitro (27). Mutations in NUP49 cause defects in nuclear protein import and nuclear RNA export (4, 12); nup49 mutations are also synthetically lethal with mutations in other nucleoporin genes (25, 12).

Last updated: 1999-07-28 Contact SGD

References cited on this page View Complete Literature Guide for NUP49
1) Wente SR, et al.  (1992) A new family of yeast nuclear pore complex proteins. J Cell Biol 119(4):705-23
2) Grandi P, et al.  (1993) Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J 12(8):3061-71
3) Hurt E, et al.  (1999) A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants. J Cell Biol 144(3):389-401
4) Sharma K, et al.  (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol Cell Biol 16(1):294-301
5) Doye V, et al.  (1994) A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution. EMBO J 13(24):6062-75
6) Stage-Zimmermann T, et al.  (2000) Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell 11(11):3777-89
7) Allen NP, et al.  (2001) Proteomic analysis of nucleoporin interacting proteins. J Biol Chem 276(31):29268-74
8) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
9) Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
10) Frey S and Gorlich D  (2009) FG/FxFG as well as GLFG repeats form a selective permeability barrier with self-healing properties. EMBO J 28(17):2554-67
11) Davis LI and Fink GR  (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
12) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
13) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
14) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
15) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
16) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
17) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
18) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
19) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
20) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
21) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
22) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
23) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
24) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
25) Wimmer C, et al.  (1992) A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1. EMBO J 11(13):5051-61
26) Iovine MK, et al.  (1995) The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor. J Cell Biol 131(6 Pt 2):1699-713
27) Schlaich NL, et al.  (1997) In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p. Mol Biol Cell 8(1):33-46
28) Fahrenkrog B, et al.  (1998) Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol 143(3):577-88