PEX14/YGL153W Summary Help

Standard Name PEX14 1
Systematic Name YGL153W
Feature Type ORF, Verified
Description Central component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p (1, 2, 3 and see Summary Paragraph)
Name Description PEroXisome related
Chromosomal Location
ChrVII:216273 to 217298 | ORF Map | GBrowse
Gene Ontology Annotations All PEX14 GO evidence and references
  View Computational GO annotations for PEX14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Classical genetics
Large-scale survey
171 total interaction(s) for 86 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 35
  • Affinity Capture-Western: 21
  • Biochemical Activity: 3
  • Co-crystal Structure: 1
  • Co-fractionation: 5
  • Co-purification: 1
  • PCA: 14
  • Protein-peptide: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 48

Genetic Interactions
  • Negative Genetic: 20
  • Positive Genetic: 3
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 341
Molecular Weight (Da) 38,420
Isoelectric Point (pI) 4.61
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:216273 to 217298 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1026 216273..217298 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003121

The biogenesis of peroxisomes requires a group of protein factors referred to as peroxins which are encoded by the PEX genes. Peroxisomal proteins are synthesized on free polyribosomes and imported posttranslationally. The transport of peroxisomal matrix proteins from the cytoplasm to the peroxisome is mediated by two peroxisome-targeting signal sequences (PTS1 and PTS2). Peroxisomal membrane proteins (PMPs) are imported independently of the matrix proteins by a distinct mechanism mediated by the membrane PTS signal (mPTS) (4, 5, 6, 7 and references therein).

Pex13p, Pex14p, and Pex17p form a docking complex that is required for PTS1 and PTS2 peroxisomal matrix proteins to associate with the peroxisomal membrane prior to import into the peroxisomal matrix (reviewed in 8, 9). Although Pex13p and Pex14p interact with both Pex5p (the receptor for PTS1) and Pex7p (the receptor for PTS2), Pex14p may be the initial docking site (2, 10, 11, 11). All members of the docking complex are localized to the cytosolic face of the peroxisomal membrane. Pex13 is an integral membrane protein with its amino and carboxy termini exposed to the cytoplasm, while Pex14 and Pex17 are peripheral membrane proteins (10, 2, 12). Therefore, interactions between the docking complex and the translocation complex comprised of Pex2p, Pex10p, and Pex12p may enable communication between both sides of the peroxisomal membrane (13).

Multiple interactions occur among the proteins of the docking complex. Pex14p interacts with Pex13p, an interaction that may be mediated by Pex5p (2, 14). Pex14p also interacts with Pex17p, even in the absence of Pex13p (12). Biochemical purification of the docking complex indicates that only 10% of Pex13p is located there, suggesting Pex13p may have additional functions (13, 15).

Of the three proteins, Pex13p and Pex14 are widely conserved but Pex17p has only been identified in ascomycetes such as S. cerevisiae and C. albicans (16). Mutations in the human Pex13 and Pex14 have been associated with the peroxisomal biogenesis disorders neonatal adrenoleukodystrophy and Zellweger syndrome (17, 18, and references therein).

Last updated: 2007-07-05 Contact SGD

References cited on this page View Complete Literature Guide for PEX14
1) Brocard C, et al.  (1997) Pex14p is a member of the protein linkage map of Pex5p. EMBO J 16(18):5491-500
2) Albertini M, et al.  (1997) Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways. Cell 89(1):83-92
3) Oeljeklaus S, et al.  (2012) Identification of core components and transient interactors of the peroxisomal importomer by dual-track stable isotope labeling with amino acids in cell culture analysis. J Proteome Res 11(4):2567-80
4) Hettema EH, et al.  (2000) Saccharomyces cerevisiae pex3p and pex19p are required for proper localization and stability of peroxisomal membrane proteins. EMBO J 19(2):223-33
5) Sacksteder KA and Gould SJ  (2000) The genetics of peroxisome biogenesis. Annu Rev Genet 34:623-652
6) Purdue PE and Lazarow PB  (2001) Peroxisome biogenesis. Annu Rev Cell Dev Biol 17:701-52
7) Fujiki Y, et al.  (2006) Import of peroxisomal membrane proteins: The interplay of Pex3p- and Pex19p-mediated interactions. Biochim Biophys Acta 1763(12):1639-46
8) Heiland I and Erdmann R  (2005) Biogenesis of peroxisomes. Topogenesis of the peroxisomal membrane and matrix proteins. FEBS J 272(10):2362-72
9) Brown LA and Baker A  (2003) Peroxisome biogenesis and the role of protein import. J Cell Mol Med 7(4):388-400
10) Girzalsky W, et al.  (1999) Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes. J Cell Biol 144(6):1151-62
11) Niederhoff K, et al.  (2005) Yeast Pex14p possesses two functionally distinct Pex5p and one Pex7p binding sites. J Biol Chem 280(42):35571-8
12) Huhse B, et al.  (1998) Pex17p of Saccharomyces cerevisiae is a novel peroxin and component of the peroxisomal protein translocation machinery. J Cell Biol 140(1):49-60
13) Agne B, et al.  (2003) Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol Cell 11(3):635-46
14) Schell-Steven A, et al.  (2005) Identification of a novel, intraperoxisomal pex14-binding site in pex13: association of pex13 with the docking complex is essential for peroxisomal matrix protein import. Mol Cell Biol 25(8):3007-18
15) Williams C and Distel B  (2006) Pex13p: Docking or cargo handling protein? Biochim Biophys Acta 1763(12):1585-91
16) Kiel JA, et al.  (2006) PEX Genes in Fungal Genomes: Common, Rare or Redundant. Traffic 7(10):1291-303
17) Bottger G, et al.  (2000) Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner. Mol Biol Cell 11(11):3963-76
18) Azevedo JE and Schliebs W  (2006) Pex14p, more than just a docking protein. Biochim Biophys Acta 1763(12):1574-84