ARO2/YGL148W Summary Help

Standard Name ARO2
Systematic Name YGL148W
Feature Type ORF, Verified
Description Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description AROmatic amino acid requiring
Chromosomal Location
ChrVII:226399 to 227529 | ORF Map | GBrowse
Gbrowse
Genetic position: -90 cM
Gene Ontology Annotations All ARO2 GO evidence and references
  View Computational GO annotations for ARO2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
210 total interaction(s) for 152 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1

Genetic Interactions
  • Negative Genetic: 164
  • Positive Genetic: 21
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 376
Molecular Weight (Da) 40,838
Isoelectric Point (pI) 7.8
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:226399 to 227529 | ORF Map | GBrowse
SGD ORF map
Genetic position: -90 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1131 226399..227529 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003116
References cited on this page View Complete Literature Guide for ARO2
1) Jones DG, et al.  (1991) Molecular cloning, characterization and analysis of the regulation of the ARO2 gene, encoding chorismate synthase, of Saccharomyces cerevisiae. Mol Microbiol 5(9):2143-52
2) Henstrand JM, et al.  (1996) Saccharomyces cerevisiae chorismate synthase has a flavin reductase activity. Mol Microbiol 22(5):859-66
3) Jones EW and Fink GR  (1982) "Regulation of amino acid and nucleotide biosynthesis in yeast." Pp.181-299 in The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, edited by Strathern JN, Jones EW and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76