MRF1/YGL143C Summary

Standard Name	MRF1 (see Nomenclature conflict Note)
Systematic Name	YGL143C
Feature Type	ORF, Verified
Description	Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability (1, 2 and see Summary Paragraph)
Name Description	Mitochondrial peptide chain Release Factor 1

Chromosomal Location	ChrVII:235958 to 234717 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All MRF1 GO evidence and references

	View Computational GO annotations for MRF1
Molecular Function
Manually curated	translation release factor activity (IDA, IGI)
Biological Process
Manually curated	mitochondrial translation (IMP) mitochondrial translational termination (IGI, IMP) translational termination (IMP)
Cellular Component
Manually curated	mitochondrion (IDA, IMP)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All MRF1 Phenotype evidence and references

Classical genetics
null	respiratory growth: absent
Large-scale survey
null	cell size: decreased glycogen accumulation: decreased competitive fitness: decreased dessication resistance: decreased hyperosmotic stress resistance: decreased mitochondrial morphology: abnormal resistance to (S)-lactic acid: decreased resistance to acetic acid: decreased resistance to cisplatin: decreased resistance to nickel sulfate: decreased resistance to sorbate: decreased resistance to ethanol: decreased respiratory growth: absent respiratory growth: decreased rate stress resistance: increased vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MRF1 Interaction evidence and references

	13 total interaction(s) for 11 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Biochemical Activity: 2 Two-hybrid: 2
Genetic Interactions	Synthetic Growth Defect: 6 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MRF1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:235958 to 234717 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1242	235958..234717	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003111

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
ETR1	Nomenclature conflict	The gene name MRF1 has been used to refer to both the mitochondrial release factor MRF1/YGL143C and the 2-enoyl thioester reductase ETR1/YBR026C

SUMMARY PARAGRAPH for MRF1

MRF1 encodes a mitochondrial peptide chain release factor that terminates translation in response to the termination codons UAA and UAG (1, 3). MRF1 has homologs in E. coli and Kluyveromyces lactis (1, 4). mrf1 mutants exhibit respiratory growth defects, and expression of the Kluyveromyces lactis MRF1 gene complements this phenotype (1, 4).

Note: ETR1/YBR026C encoding a 2-enoyl thioester reductase has also been referred to as MRF1 in the literature.

Last updated: 2000-06-09

References cited on this page View Complete Literature Guide for MRF1

1)	Pel HJ, et al. (1992) The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects. Nucleic Acids Res 20(23):6339-46
2)	Towpik J, et al. (2004) Mutations in the yeast mrf1 gene encoding mitochondrial release factor inhibit translation on mitochondrial ribosomes. J Biol Chem 279(14):14096-103
3)	Askarian-Amiri ME, et al. (2000) Functional characterization of yeast mitochondrial release factor 1. J Biol Chem 275(23):17241-8
4)	Pel HJ, et al. (1996) The nuclear Kluyveromyces lactis MRF1 gene encodes a mitochondrial class I peptide chain release factor that is important for cell viability. Curr Genet 30(1):19-28