HUL5/YGL141W Summary Help

Standard Name HUL5 1
Systematic Name YGL141W
Feature Type ORF, Verified
Description Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD (1, 2, 3, 4)
Name Description Hect Ubiquitin Ligase 1
Chromosomal Location
ChrVII:238353 to 241085 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All HUL5 GO evidence and references
  View Computational GO annotations for HUL5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
30 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 1
  • Co-purification: 2
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 6
  • Phenotypic Suppression: 3
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 910
Molecular Weight (Da) 105,564
Isoelectric Point (pI) 6.4
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:238353 to 241085 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2733 238353..241085 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003109
References cited on this page View Complete Literature Guide for HUL5
1) Wang G, et al.  (1999) Functional domains of the Rsp5 ubiquitin-protein ligase. Mol Cell Biol 19(1):342-52
2) Crosas B, et al.  (2006) Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities. Cell 127(7):1401-13
3) Kohlmann S, et al.  (2008) Ubiquitin Ligase Hul5 Is Required for Fragment-specific Substrate Degradation in Endoplasmic Reticulum-associated Degradation. J Biol Chem 283(24):16374-83
4) Aviram S and Kornitzer D  (2010) The ubiquitin ligase Hul5 promotes proteasomal processivity. Mol Cell Biol 30(4):985-94