SEC27/YGL137W Summary Help

Standard Name SEC27 1
Systematic Name YGL137W
Feature Type ORF, Verified
Description Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP (1, 2, 3)
Name Description SECretory
Chromosomal Location
ChrVII:249869 to 252738 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SEC27 GO evidence and references
  View Computational GO annotations for SEC27
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Resources
Classical genetics
conditional
null
repressible
Large-scale survey
null
reduction of function
repressible
Resources
393 total interaction(s) for 244 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 142
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 9
  • PCA: 31
  • Protein-peptide: 3
  • Protein-RNA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 6
  • Negative Genetic: 172
  • Positive Genetic: 9
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 889
Molecular Weight (Da) 99,444
Isoelectric Point (pI) 4.46
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:249869 to 252738 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..18 249869..249886 2011-02-03 1996-07-31
Intron 19..218 249887..250086 2011-02-03 1996-07-31
CDS 219..2870 250087..252738 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003105
References cited on this page View Complete Literature Guide for SEC27
1) Duden R, et al.  (1994) Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. J Biol Chem 269(39):24486-95
2) Letourneur F, et al.  (1994) Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum. Cell 79(7):1199-207
3) Eugster A, et al.  (2004) The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol Biol Cell 15(3):1011-23