NAB2/YGL122C Summary Help

Standard Name NAB2 1
Systematic Name YGL122C
Feature Type ORF, Verified
Description Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; involved in forming export-competent mRNPs in the nucleus; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Nuclear polyAdenylated RNA-Binding 1
Chromosomal Location
ChrVII:280523 to 278946 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NAB2 GO evidence and references
  View Computational GO annotations for NAB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 76 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
overexpression
Resources
2693 total interaction(s) for 2582 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 44
  • Affinity Capture-RNA: 2541
  • Affinity Capture-Western: 27
  • Biochemical Activity: 5
  • Co-crystal Structure: 1
  • Co-localization: 1
  • PCA: 2
  • Reconstituted Complex: 14
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 1
  • Dosage Rescue: 7
  • Negative Genetic: 3
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 3
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 525
Molecular Weight (Da) 58,321
Isoelectric Point (pI) 8.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:280523 to 278946 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1578 280523..278946 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003090
References cited on this page View Complete Literature Guide for NAB2
1) Anderson JT, et al.  (1993) NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability. Mol Cell Biol 13(5):2730-41
2) Wilson SM, et al.  (1994) Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. J Cell Biol 127(5):1173-84
3) Truant R, et al.  (1998) Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol Cell Biol 18(3):1449-58
4) Green DM, et al.  (2002) Nab2p is required for poly(A) RNA export in Saccharomyces cerevisiae and is regulated by arginine methylation via Hmt1p. J Biol Chem 277(10):7752-60
5) Hector RE, et al.  (2002) Dual requirement for yeast hnRNP Nab2p in mRNA poly(A) tail length control and nuclear export. EMBO J 21(7):1800-10
6) Roth KM, et al.  (2005) The nuclear exosome contributes to autogenous control of NAB2 mRNA levels. Mol Cell Biol 25(5):1577-85
7) Fasken MB, et al.  (2008) Functional Significance of the Interaction between the mRNA-binding Protein, Nab2, and the Nuclear Pore-associated Protein, Mlp1, in mRNA Export. J Biol Chem 283(40):27130-43
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Brockmann C, et al.  (2012) Structural basis for polyadenosine-RNA binding by Nab2 Zn fingers and its function in mRNA nuclear export. Structure 20(6):1007-18