Standard Name	PRP43 1
Systematic Name	YGL120C
Feature Type	ORF, Verified
Description	RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome (1, 2, 3, 4) Also known as: JA1
Name Description	Pre-mRNA Processing 1

Chromosomal Location	ChrVII:283937 to 281634 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for PRP43
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (IDA, IMP)
Biological Process
Manually curated	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of SSU-rRNA (IGI) maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) ribosomal large subunit biogenesis (IMP) rRNA processing (IMP) spliceosomal complex disassembly (IDA, IGI)
Cellular Component
Manually curated	post-mRNA release spliceosomal complex (IMP)
High-throughput	90S preribosome (IDA) mitochondrion (IDA)

Classical genetics
reduction of function	pre-mRNA accumulation: increased 20S pre-rRNA accumulation: decreased 27S pre-rRNA accumulation: decreased cold sensitivity: increased heat sensitivity: increased
Large-scale survey
conditional	resistance to benomyl: decreased
null	haploproficient inviable
overexpression	vegetative growth: decreased rate
reduction of function	competitive fitness: increased resistance to methyl methanesulfonate: decreased telomere length: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	284 total interaction(s) for 175 unique genes/features.
Physical Interactions	Affinity Capture-MS: 237 Affinity Capture-RNA: 1 Affinity Capture-Western: 10 Biochemical Activity: 1 Co-fractionation: 3 PCA: 11 Reconstituted Complex: 2 Two-hybrid: 8
Genetic Interactions	Dosage Lethality: 2 Positive Genetic: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 6 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:283937 to 281634 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2304 283937..281634 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003088