MLC1/YGL106W Summary Help

MLC1 BASIC INFORMATION

Standard Name MLC1 1
Systematic Name YGL106W
Feature Type ORF, Verified
Description Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition (1, 2, 3 and see Summary Paragraph)
Name Description Myosin Light Chain 1
GO Annotations All MLC1 GO evidence and references
    View Computational GO annotations for MLC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All MLC1 Phenotype details and references
Classical genetics
null
Large-scale survey
repressible
Interactions MLC1 All interactions details and references
102 total interaction(s) for 61 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 70
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 11
  • Co-crystal Structure: 3
  • Co-localization: 1
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 5
Genetic Interactions
  • Dosage Rescue: 4
  • Phenotypic Enhancement: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2
Sequence Information
ChrVII:306563 to 307012 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..450 306563..307012 2004-07-20 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003074

MLC1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for MLC1

SUMMARY PARAGRAPH for MLC1

Mlc1p an essential light chain (ELC) for Myo1p, binding to its IQ1 motif, while Mlc2p is a regulatory light chain (RLC) for Myo1p (3, 4). Mlc1p is also a light chain for the type V myosin Myo2p (1) and the IQGAP-like protein, Iqg1p (5).

MLC1 is an essential gene and exhibits haploinsufficiency (1). Mlc1p localizes to the bud neck and its interactions with Myo1p and Iqg1p play a role in actin ring formation and promote targeted membrane deposition at the bud neck (3) during cytokinesis. Inviable mlc1 null mutant cells show a defect in cytokinesis, resulting in enlarged, multinucleate cells (1), a phenotype similar to cells deleted for IQG1 (6). Disruption of the Mlc1p-Myo1p or Mlc1p-Myo2p interaction does not prevent Mlc1p targeting to the bud neck. However, disrupting the Mlc1p-Iqg1p interaction by depleting Mlc1p prevents localization of Iqg1p to the bud neck which in turn prevents actin localization to the bud neck and actomyosin ring formation (4, 5). Thus, the Mlc1p-Iqg1p interaction plays a major role in cytokinesis. Mlc1p associates with secretory vesicles and forms a complex(es) with Sec4p and Myo2p and is required for vesicle targeting during cytokinesis (2).

Compared to the RLCs, the sequence of the ELCs are better conserved. Mlc1p is similar to S. pombe ELC (CDC4), D. discoideum ELC, chicken light chain (MLE1 gene) and Acanthamoeba myosin light chain (3 and references therein).

Last updated: 2007-05-08

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MLC1]

1) Stevens RC and Davis TN  (1998) Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae. J Cell Biol 142(3):711-22
2) Wagner W, et al.  (2002) Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p. EMBO J 21(23):6397-408
3) Luo J, et al.  (2004) Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J Cell Biol 165(6):843-55
4) Boyne JR, et al.  (2000) Yeast myosin light chain, Mlc1p, interacts with both IQGAP and class II myosin to effect cytokinesis. J Cell Sci 113 Pt 24:4533-43
5) Shannon KB and Li R  (2000) A myosin light chain mediates the localization of the budding yeast IQGAP-like protein during contractile ring formation. Curr Biol 10(12):727-30
6) Epp JA and Chant J  (1997) An IQGAP-related protein controls actin-ring formation and cytokinesis in yeast. Curr Biol 7(12):921-9