SRM1/YGL097W Summary Help

Standard Name SRM1 1
Systematic Name YGL097W
Alias MTR1 2 , PRP20 3 , TSM437 4
Feature Type ORF, Verified
Description Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p (1, 5, 6, 7)
Name Description Suppressor of Receptor Mutations 1
Chromosomal Location
ChrVII:321782 to 323230 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SRM1 GO evidence and references
  View Computational GO annotations for SRM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
conditional
overexpression
Large-scale survey
conditional
null
reduction of function
unspecified
Resources
116 total interaction(s) for 81 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-localization: 1
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 12
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 7
  • Negative Genetic: 29
  • Phenotypic Suppression: 2
  • Positive Genetic: 8
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 9
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 482
Molecular Weight (Da) 53,013
Isoelectric Point (pI) 6.02
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:321782 to 323230 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1449 321782..323230 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003065
References cited on this page View Complete Literature Guide for SRM1
1) Clark KL and Sprague GF Jr  (1989) Yeast pheromone response pathway: characterization of a suppressor that restores mating to receptorless mutants. Mol Cell Biol 9(6):2682-94
2) Kadowaki T, et al.  (1994) Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants. J Cell Biol 126(3):649-59
3) Vijayraghavan U, et al.  (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 3(8):1206-16
4) Clark KL, et al.  (1991) The yeast SRM1 protein and human RCC1 protein share analogous functions. Cell Regul 2(10):781-92
5) Akhtar N, et al.  (2001) Functional analysis of the yeast Ran exchange factor Prp20p: in vivo evidence for the RanGTP gradient model. Mol Genet Genomics 265(5):851-64
6) Clement M, et al.  (2001) Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae. Mol Genet Genomics 266(1):20-7
7) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64