NUP145/YGL092W Summary Help

NUP145 BASIC INFORMATION

Standard Name NUP145
Systematic Name YGL092W
Alias RAT10
Feature Type ORF, Verified
Description Essential nucleoporin, catalyzes its own cleavage in vivo to generate a C-terminal fragment that assembles into the Nup84p subcomplex of the nuclear pore complex, and an N-terminal fragment of unknown function that is homologous to Nup100p (1, 2 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP145 GO evidence and references
    View Computational GO annotations for NUP145
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All NUP145 Phenotype details and references
Classical genetics
repressible
Large-scale survey
null
Interactions NUP145 All interactions details and references
95 total interaction(s) for 31 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 46
  • Affinity Capture-Western: 12
  • Co-purification: 2
  • FRET: 6
  • PCA: 1
  • Reconstituted Complex: 12
  • Two-hybrid: 4
Genetic Interactions
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 7
Sequence Information
ChrVII:337909 to 341862 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3954 337909..341862 2004-07-20 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003060

NUP145 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for NUP145

SUMMARY PARAGRAPH for NUP145

NUP145 encodes an essential nuclear pore protein (3). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 3, 4, 5, 6). The structure of the vertebrate NPC has been studied extensively; recent reviews include 7, 8, 9, and 10. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (11, 12). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 3, 4, 13, 14, 15). The N- and C-terminal domains of Nup145p are functionally distinct (16, 17, 2) and are cleaved in vivo (17, 2), though the cleavage is not essential for Nup145p function (17). The N-terminal half of Nup145p contains multiple repeats of the amino acids GLFG (18, 3) and is similar to Nup100p and Nup116p (19, 3). The C-terminal half assembles into the NPC subcomplex containing Nup84p and several other nucleoporins (2, 20, 3). Mutations in NUP145 cause defects in nuclear protein import and nuclear RNA export (21, 22, 3, 17, 16). nup145 mutations are also synthetically lethal with mutations in several other nucleoporin genes (3). A related nucleoporin, Nup98, has been identified in vertebrates (3).

Last updated: 1999-08-10

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP145]

1) Teixeira MT, et al.  (1999) Self-catalyzed cleavage of the yeast nucleoporin Nup145p precursor. J Biol Chem 274(45):32439-44
2) Teixeira MT, et al.  (1997) Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins. EMBO J 16(16):5086-97
3) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
4) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
6) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
7) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
8) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
9) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
10) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
11) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
12) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
13) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
14) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
15) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
16) Dockendorff TC, et al.  (1997) C-terminal truncations of the yeast nucleoporin Nup145p produce a rapid temperature-conditional mRNA export defect and alterations to nuclear structure. Mol Cell Biol 17(2):906-20
17) Emtage JL, et al.  (1997) Defining the essential functional regions of the nucleoporin Nup145p. J Cell Sci 110 ( Pt 7):911-25
18) Wente SR, et al.  (1992) A new family of yeast nuclear pore complex proteins. J Cell Biol 119(4):705-23
19) Wente SR and Blobel G  (1994) NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J Cell Biol 125(5):955-69
20) Siniossoglou S, et al.  (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84(2):265-75
21) Fabre E, et al.  (1994) Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif. Cell 78(2):275-89
22) Sharma K, et al.  (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol Cell Biol 16(1):294-301