DUO1/YGL061C Summary Help

DUO1 BASIC INFORMATION

Standard Name DUO1
Systematic Name YGL061C
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis (1, 2, 3, 4)
Name Description Death Upon Overproduction 5
GO Annotations All DUO1 GO evidence and references
    View Computational GO annotations for DUO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All DUO1 Phenotype details and references
Large-scale survey
null
Interactions DUO1 All interactions details and references
75 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-Western: 9
  • Biochemical Activity: 1
  • Co-purification: 5
  • PCA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 27
Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3
Sequence Information
ChrVII:389714 to 388971 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..744 389714..388971 2004-07-20 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003029

DUO1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for DUO1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for DUO1]

1) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
2) Miranda JJ, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
3) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
4) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9
5) Hofmann C, et al.  (1998) Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function. J Cell Biol 143(4):1029-40