PRM8/YGL053W Summary

PRM8/YGL053W Summary

Standard Name	PRM8 1
Systematic Name	YGL053W
Feature Type	ORF, Verified
Description	Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family (1, 2, 3, 4)
Name Description	Pheromone-Regulated Membrane protein 1

Chromosomal Location	ChrVII:402589 to 403302 \| ORF Map \| GBrowse

Gene Ontology Annotations All PRM8 GO evidence and references

	View Computational GO annotations for PRM8
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	biological_process unknown (ND)
Cellular Component
Manually curated	endoplasmic reticulum (IDA, IMP) integral to membrane (ISS) plasma membrane (IMP)

Mutant phenotypes All PRM8 Phenotype evidence and references

Large-scale survey
null	competitive fitness: increased haploinsufficient heat sensitivity: increased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PRM8 Interaction evidence and references

	26 total interaction(s) for 26 unique genes/features.
Physical Interactions	Affinity Capture-Western: 1 Co-purification: 1 PCA: 4 Two-hybrid: 9
Genetic Interactions	Negative Genetic: 8 Positive Genetic: 1 Synthetic Growth Defect: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PRM8 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:402589 to 403302 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..714	402589..403302	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003021

References cited on this page View Complete Literature Guide for PRM8

1)	Heiman MG and Walter P (2000) Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating. J Cell Biol 151(3):719-30
2)	Feuermann M, et al. (1997) The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes. Yeast 13(9):861-9
3)	Sandmann T, et al. (2003) Suppression of coatomer mutants by a new protein family with COPI and COPII binding motifs in Saccharomyces cerevisiae. Mol Biol Cell 14(8):3097-113
4)	Poirey R, et al. (2002) Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability. Microbiology 148(Pt 7):2111-23