TIF4632/YGL049C Summary Help

Standard Name TIF4632 1
Systematic Name YGL049C
Feature Type ORF, Verified
Description Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description Translation Initiation Factor 1
Gene Product Alias eIF4G2 5
Chromosomal Location
ChrVII:409604 to 406860 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TIF4632 GO evidence and references
  View Computational GO annotations for TIF4632
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
136 total interaction(s) for 71 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 72
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 13
  • PCA: 4
  • Reconstituted Complex: 15
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 12
  • Negative Genetic: 4
  • Phenotypic Suppression: 3
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 914
Molecular Weight (Da) 103,898
Isoelectric Point (pI) 7.69
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:409604 to 406860 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2745 409604..406860 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003017
References cited on this page View Complete Literature Guide for TIF4632
1) Goyer C, et al.  (1993) TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function. Mol Cell Biol 13(8):4860-74
2) Tarun SZ Jr, et al.  (1997) Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation. Proc Natl Acad Sci U S A 94(17):9046-51
3) Dominguez D, et al.  (1999) Interaction of translation initiation factor eIF4G with eIF4A in the yeast Saccharomyces cerevisiae. J Biol Chem 274(38):26720-6
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Neff CL and Sachs AB  (1999) Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally. Mol Cell Biol 19(8):5557-64