RIM8/YGL045W Summary

RIM8/YGL045W Summary

Standard Name	RIM8 1
Systematic Name	YGL045W
Alias	PAL3 2 , YGL046W , ART9 3
Feature Type	ORF, Verified
Description	Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family (2, 3, 4, 5, 6)
Name Description	Regulator of IME2 1

Chromosomal Location	ChrVII:414102 to 415730 \| ORF Map \| GBrowse

Gene Ontology Annotations All RIM8 GO evidence and references

	View Computational GO annotations for RIM8
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	invasive growth in response to glucose limitation (IMP) meiosis (IMP) protein processing (IMP) ubiquitin-dependent endocytosis (ISA)
Cellular Component
Manually curated	internal side of plasma membrane (IDA)

Mutant phenotypes All RIM8 Phenotype evidence and references

Classical genetics
conditional	cold sensitivity: increased
null	resistance to BPS: decreased
unspecified	sporulation efficiency: decreased
Large-scale survey
null	acid pH resistance: decreased competitive fitness: decreased endocytosis: decreased filamentous growth: decreased haploinsufficient hyperosmotic stress resistance: decreased invasive growth: absent ionic stress resistance: decreased Fus-Mid-GFP distribution: abnormal resistance to nickel sulfate: increased resistance to sirolimus: decreased resistance to tunicamycin: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to methyl methanesulfonate: decreased resistance to wortmannin: normal resistance to BPS: decreased resistance to lovastatin: decreased resistance to dihydromotuporamine C: decreased resistance to calcium dichloride: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased sporulation efficiency: decreased toxin resistance: decreased vacuolar morphology: abnormal vegetative growth: absent viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All RIM8 Interaction evidence and references

	287 total interaction(s) for 214 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Reconstituted Complex: 3 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 208 Phenotypic Enhancement: 1 Positive Genetic: 29 Synthetic Growth Defect: 18 Synthetic Lethality: 12 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All RIM8 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:414102 to 415730 | |

This feature contains embedded feature(s): YGL046W

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 2003-01-09

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1629	414102..415730	2011-02-03	2003-01-09

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003013

References cited on this page View Complete Literature Guide for RIM8

1)	Su SS and Mitchell AP (1993) Identification of functionally related genes that stimulate early meiotic gene expression in yeast. Genetics 133(1):67-77
2)	Treton B, et al. (2000) Ambient pH signalling in ascomycetous yeasts involves homologues of the Aspergillus nidulans genes palF and paIH. Mol Gen Genet 263(3):505-13
3)	Lin CH, et al. (2008) Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell 135(4):714-25
4)	Li W and Mitchell AP (1997) Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth. Genetics 145(1):63-73
5)	Snoek IS and Steensma HY (2006) Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome. FEMS Yeast Res 6(3):393-403
6)	Herrador A, et al. (2010) Recruitment of the ESCRT machinery to a putative seven-transmembrane-domain receptor is mediated by an arrestin-related protein. Mol Cell Biol 30(4):897-907