RNA15/YGL044C Summary Help

Standard Name RNA15
Systematic Name YGL044C
Feature Type ORF, Verified
Description Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping (1, 2, 3, 4, 5)
Name Description RNA synthesis
Chromosomal Location
ChrVII:417036 to 416146 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RNA15 GO evidence and references
  View Computational GO annotations for RNA15
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
Resources
183 total interaction(s) for 115 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-Western: 23
  • Co-crystal Structure: 1
  • Co-fractionation: 3
  • Co-purification: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 64
  • Phenotypic Suppression: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 10
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 296
Molecular Weight (Da) 32,790
Isoelectric Point (pI) 4.75
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:417036 to 416146 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..891 417036..416146 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003012
References cited on this page View Complete Literature Guide for RNA15
1) Gross S and Moore C  (2001) Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc Natl Acad Sci U S A 98(11):6080-5
2) Gross S and Moore CL  (2001) Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation. Mol Cell Biol 21(23):8045-55
3) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
4) Medler S, et al.  (2011) Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping. J Biol Chem 286(39):33709-18
5) Gordon J, et al.  (2011) Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology. Biochemistry 50(47):10203-14