DST1/YGL043W Summary Help

Standard Name DST1 1
Systematic Name YGL043W
Alias PPR2 2
Feature Type ORF, Verified
Description General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress (3, 4, 5, 6, 7)
Name Description DNA Strand Transfer 1
Gene Product Alias P37 8 , S-II 9 , SII 10 , TFIIS 10
Chromosomal Location
ChrVII:417483 to 418412 | ORF Map | GBrowse
Genetic position: 24 cM
Gene Ontology Annotations All DST1 GO evidence and references
  View Computational GO annotations for DST1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
Large-scale survey
432 total interaction(s) for 274 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 193
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 7
  • Positive Genetic: 28
  • Synthetic Growth Defect: 68
  • Synthetic Lethality: 53
  • Synthetic Rescue: 17

Expression Summary
Length (a.a.) 309
Molecular Weight (Da) 34,843
Isoelectric Point (pI) 9.38
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:417483 to 418412 | ORF Map | GBrowse
Genetic position: 24 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..930 417483..418412 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003011
References cited on this page View Complete Literature Guide for DST1
1) Clark AB, et al.  (1991) Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha. Mol Cell Biol 11(5):2576-82
2) Hubert JC, et al.  (1983) Complete sequence of a eukaryotic regulatory gene. EMBO J 2(11):2071-3
3) Davie JK and Kane CM  (2000) Genetic interactions between TFIIS and the Swi-Snf chromatin-remodeling complex. Mol Cell Biol 20(16):5960-73
4) Wu J, et al.  (1996) In vitro characterization of mutant yeast RNA polymerase II with reduced binding for elongation factor TFIIS. Proc Natl Acad Sci U S A 93(21):11552-7
5) Olmsted VK, et al.  (1998) Yeast transcript elongation factor (TFIIS), structure and function. I: NMR structural analysis of the minimal transcriptionally active region. J Biol Chem 273(35):22589-94
6) Ubukata T, et al.  (2003) Cleavage, but not read-through, stimulation activity is responsible for three biologic functions of transcription elongation factor S-II. J Biol Chem 278(10):8580-5
7) Gomez-Herreros F, et al.  (2012) TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress. Nucleic Acids Res 40(14):6508-19
8) Sawadogo M, et al.  (1980) Similar binding site for P37 factor on yeast RNA polymerases A and B. Biochem Biophys Res Commun 96(1):258-64
9) Nakanishi T, et al.  (1992) Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae. J Biol Chem 267(19):13200-4
10) Davies CJ, et al.  (1990) Homologue of TFIIS in yeast. Nature 345(6273):298