MIG1/YGL035C Summary Help

MIG1 BASIC INFORMATION

Standard Name MIG1 1
Systematic Name YGL035C
Alias CAT4 , SSN1 2 , TDS22
Feature Type ORF, Verified
Description Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase (1, 3, 4, 5 and see Summary Paragraph)
Name Description Multicopy Inhibitor of GAL gene expression 1
GO Annotations All MIG1 GO evidence and references
    View Computational GO annotations for MIG1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All MIG1 Phenotype details and references
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Interactions MIG1 All interactions details and references
95 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Biochemical Activity: 11
  • Reconstituted Complex: 2
  • Two-hybrid: 15
Genetic Interactions
  • Dosage Rescue: 16
  • Phenotypic Enhancement: 19
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 1
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 12
Sequence Information
ChrVII:433067 to 431553 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -27 cM
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1515 433067..431553 2004-07-20 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003003

MIG1 RESOURCES

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Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for MIG1

SUMMARY PARAGRAPH for MIG1

MIG1 was first identified as a Multicopy Inhibitor of Galactose gene expression (1). Mig1p is a Zinc-finger protein, of the Cys2His2 type, that binds specifically to DNA with a GC-rich consensus sequence and a flanking AT sequence (3). It is thought that a major function of Mig1p is to repress the transcription of genes whose expression is shut off when glucose is present, such as those encoding enzymes for utilization of the sugars maltose, sucrose, or galactose (6, 4). In addition to Mig1p, other Cys2His2 type zinc finger containing DNA-binding repressor proteins such as Mig2p and Mig3p are also involved (7, 6, 4). It has also been shown that in some contexts, Mig1p functions as a transcriptional activator (4, 5).

According to the long-standing recruitment model, during repressing conditions, i.e. in the presence of glucose, cytoplasmically located Mig1p is dephosphorylated by the Reg1p-Glc7p protein phosphatase complex and then imported into the nucleus (4). In the nucleus, it binds to promoters of glucose-repressed genes where it recruits the Cyc8p-Tup1p corepressor complex (4). When cells become limited for glucose, Mig1p is phosphorylated by the Snf1 kinase complex, composed of the Snf1 kinase catalytic subunit, the gamma subunit Snf4p, and a beta subunit encoded by SIP1, SIP2, or GAL83 (5). Upon phosphorylation, Mig1p is exported from the nucleus by the nuclear exportin Msn5p (4). However, nuclear export does not completely account for derepression of glucose-repressed genes; thus in this model least one other mechanism is involved in the inactiviation of the Mig1p repressor (4).

A newer alternative model, called reverse recruitment, postulates that promoters regulated by many transcription factors, including Mig1p, are recruited to "Gene Expression Machines", or GEMs, which are located at the nuclear periphery and are associated with a nuclear pore complex and other complexes involved in mRNA processing and export (5). In this model, DNA binding factors do not diffuse through the nucleus, but rather bind to the promoters at the nuclear pore in both repressing and activating conditions. Phosphorylation of DNA-bound regulators, such as Mig1p, may result in subtle conformational changes which control whether the factor functions as a repressor or an activator (5).

Last updated: 2007-02-16

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MIG1]

1) Nehlin JO and Ronne H  (1990) Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins. EMBO J 9(9):2891-8
2) Carlson M, et al.  (1984) A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast. Genetics 107(1):19-32
3) Lundin M, et al.  (1994) Importance of a flanking AT-rich region in target site recognition by the GC box-binding zinc finger protein MIG1. Mol Cell Biol 14(3):1979-85
4) Schuller HJ  (2003) Transcriptional control of nonfermentative metabolism in the yeast Saccharomyces cerevisiae. Curr Genet 43(3):139-60
5) Santangelo GM  (2006) Glucose signaling in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 70(1):253-82
6) Carlson M  (1999) Glucose repression in yeast. Curr Opin Microbiol 2(2):202-7
7) Lutfiyya LL, et al.  (1998) Characterization of three related glucose repressors and genes they regulate in Saccharomyces cerevisiae. Genetics 150(4):1377-91