CWH41/YGL027C Summary Help

Standard Name CWH41 1
Systematic Name YGL027C
Alias GLS1 , DER7 2
Feature Type ORF, Verified
Description Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress (1, 2, 3, 4 and see Summary Paragraph)
Name Description Calcofluor White Hypersensitive 1
Chromosomal Location
ChrVII:446143 to 443642 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CWH41 GO evidence and references
  View Computational GO annotations for CWH41
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
unspecified
Large-scale survey
null
overexpression
Resources
268 total interaction(s) for 133 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Co-purification: 1
  • PCA: 2

Genetic Interactions
  • Negative Genetic: 176
  • Phenotypic Enhancement: 30
  • Phenotypic Suppression: 8
  • Positive Genetic: 8
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 9
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 833
Molecular Weight (Da) 96,506
Isoelectric Point (pI) 4.81
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:446143 to 443642 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2502 446143..443642 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002995
SUMMARY PARAGRAPH for CWH41

During N-linked glycosylation of proteins, oligosaccharide chains are assembled on the carrier molecule dolichyl pyrophosphate in the following order: 2 molecules of N-acetylglucosamine (GlcNAc), 9 molecules of mannose, and 3 molecules of glucose. These 14-residue oligosaccharide cores are then transferred to asparagine residues on nascent polypeptide chains in the endoplasmic reticulum (ER). As proteins progress through the Golgi apparatus, the oligosaccharide cores are modified by trimming and extension to generate a diverse array of glycosylated proteins (reviewed in 5, 6).

CWH41, also known as GLS1, encodes glucosidase I (E.C. 3.2.1.106), an integral membrane protein of the ER (4) which removes the terminal glucose from core oligosaccharides immediately after they are transferred to proteins (7), thereby reversing the reaction catalyzed by Die2p. Although cwh41 mutants are deficient in trimming glucose from core oligosaccharides (3, 8), the downstream effect is usually not severe. Mutants grow, mate, and sporulate normally (4, 8), but have reduced levels of beta-1,6-glucan in the cell wall (4), causing hypersensitivity to the drug Calcofluor White (4, 1) and resistance to yeast K1 killer toxin (4, 1). Proteins with extraneous glucose moieties otherwise mature and are secreted normally (8), although there is a report of failure to degrade certain proteins in the ER (2). Deficiency of the human homolog, GCS1 (OMIM), causes the congenital disorder of glycosylation CDG-IIb (OMIM).

Last updated: 2005-07-06 Contact SGD

References cited on this page View Complete Literature Guide for CWH41
1) Ram AF, et al.  (1994) A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white. Yeast 10(8):1019-30
2) Hitt R and Wolf DH  (2004) DER7, encoding alpha-glucosidase I is essential for degradation of malfolded glycoproteins of the endoplasmic reticulum. FEMS Yeast Res 4(8):815-20
3) Romero PA, et al.  (1997) The yeast CWH41 gene encodes glucosidase I. Glycobiology 7(7):997-1004
4) Jiang B, et al.  (1996) CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly. J Bacteriol 178(4):1162-71
5) Herscovics A and Orlean P  (1993) Glycoprotein biosynthesis in yeast. FASEB J 7(6):540-50
6) Burda P and Aebi M  (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426(2):239-57
7) Simons JF, et al.  (1998) Cell wall 1,6-beta-glucan synthesis in Saccharomyces cerevisiae depends on ER glucosidases I and II, and the molecular chaperone BiP/Kar2p. EMBO J 17(2):396-405
8) Esmon B, et al.  (1984) Early steps in processing of yeast glycoproteins. J Biol Chem 259(16):10322-7