PGD1/YGL025C Summary Help

PGD1 BASIC INFORMATION

Standard Name PGD1
Systematic Name YGL025C
Alias HRS1 , MED3
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor (1, 2, 3, 4, 5)
Name Description PolyGlutamine Domain 6
GO Annotations All PGD1 GO evidence and references
    View Computational GO annotations for PGD1
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Mutant Phenotype All PGD1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions PGD1 All interactions details and references
238 total interaction(s) for 148 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 56
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 19
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-purification: 30
  • Far Western: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 11
Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 86
  • Phenotypic Suppression: 8
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 10
  • Synthetic Rescue: 3
Sequence Information
ChrVII:449962 to 448769 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 2003-09-22
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1194 449962..448769 2004-07-20 2003-09-22
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002993

PGD1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
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  • Protein Info & Structure
  • Localization Resources
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  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for PGD1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PGD1]

1) Papamichos-Chronakis M, et al.  (2000) Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme. J Biol Chem 275(12):8397-403
2) Lee M, et al.  (2000) Genetic analysis of the role of Pol II holoenzyme components in repression by the Cyc8-Tup1 corepressor in yeast. Genetics 155(4):1535-42
3) Reeves WM and Hahn S  (2003) Activator-independent functions of the yeast mediator sin4 complex in preinitiation complex formation and transcription reinitiation. Mol Cell Biol 23(1):349-58
4) Myers LC, et al.  (1999) Mediator protein mutations that selectively abolish activated transcription. Proc Natl Acad Sci U S A 96(1):67-72
5) Guglielmi B, et al.  (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91
6) Brohl S, et al.  (1994) A new nuclear suppressor system for a mitochondrial RNA polymerase mutant identifies an unusual zinc-finger protein and a polyglutamine domain protein in Saccharomyces cerevisiae. Yeast 10(6):719-31