PGD1/YGL025C Summary Help

Standard Name PGD1 1
Systematic Name YGL025C
Alias HRS1 , MED3
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor (2, 3, 4, 5, 6)
Name Description PolyGlutamine Domain 1
Chromosomal Location
ChrVII:449957 to 448764 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PGD1 GO evidence and references
  View Computational GO annotations for PGD1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 20 genes
Regulators 4 genes
Classical genetics
Large-scale survey
288 total interaction(s) for 185 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 57
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 24
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-purification: 31
  • Far Western: 1
  • PCA: 3
  • Reconstituted Complex: 4
  • Two-hybrid: 33

Genetic Interactions
  • Dosage Growth Defect: 5
  • Dosage Rescue: 1
  • Negative Genetic: 84
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 6
  • Positive Genetic: 8
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 9
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 397
Molecular Weight (Da) 43,079
Isoelectric Point (pI) 9.13
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:449957 to 448764 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1194 449957..448764 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002993
References cited on this page View Complete Literature Guide for PGD1
1) Brohl S, et al.  (1994) A new nuclear suppressor system for a mitochondrial RNA polymerase mutant identifies an unusual zinc-finger protein and a polyglutamine domain protein in Saccharomyces cerevisiae. Yeast 10(6):719-31
2) Papamichos-Chronakis M, et al.  (2000) Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme. J Biol Chem 275(12):8397-403
3) Lee M, et al.  (2000) Genetic analysis of the role of Pol II holoenzyme components in repression by the Cyc8-Tup1 corepressor in yeast. Genetics 155(4):1535-42
4) Reeves WM and Hahn S  (2003) Activator-independent functions of the yeast mediator sin4 complex in preinitiation complex formation and transcription reinitiation. Mol Cell Biol 23(1):349-58
5) Myers LC, et al.  (1999) Mediator protein mutations that selectively abolish activated transcription. Proc Natl Acad Sci U S A 96(1):67-72
6) Guglielmi B, et al.  (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91