Standard Name	PUF4 1
Systematic Name	YGL014W
Alias	YGL023 2
Feature Type	ORF, Verified
Description	Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors (3)
Name Description	PUmilio-homology domain Family

Chromosomal Location	ChrVII:466141 to 468807 | ORF Map | GBrowse

	View Computational GO annotations for PUF4
Molecular Function
Manually curated	mRNA binding (IDA, ISS)
High-throughput	mRNA binding (IDA)
Biological Process
Manually curated	negative regulation of chromatin silencing involved in replicative cell aging (IGI, IMP) negative regulation of translation (IDA) nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (IGI) protein localization (IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Classical genetics
overexpression	budding: abnormal cell cycle progression in G1 phase: abnormal
Large-scale survey
null	cell cycle progression in G1 phase: increased duration competitive fitness: decreased endocytosis: decreased filamentous growth: decreased haploinsufficient invasive growth: absent resistance to hygromycin B: increased resistance to spermine: increased resistance to tetramethylammonium: increased resistance to bleomycin: increased resistance to fenpropimorph: increased resistance to tunicamycin: increased resistance to cycloheximide: decreased resistance to camptothecin: increased resistance to glycolaldehyde: decreased starvation resistance: decreased vegetative growth: decreased rate viable
overexpression	inviable vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	133 total interaction(s) for 127 unique genes/features.
Physical Interactions	Affinity Capture-MS: 48 Affinity Capture-RNA: 3 Affinity Capture-Western: 3 Biochemical Activity: 3 Co-crystal Structure: 1 PCA: 8 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 50 Phenotypic Enhancement: 1 Positive Genetic: 9 Synthetic Growth Defect: 3 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:466141 to 468807 | ORF Map | GBrowse
	This feature contains embedded feature(s): YGL014C-A
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2667 466141..468807 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002982