PUF4/YGL014W Summary Help

Standard Name PUF4 1
Systematic Name YGL014W
Alias YGL023 2
Feature Type ORF, Verified
Description Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors (3)
Name Description PUmilio-homology domain Family
Chromosomal Location
ChrVII:466141 to 468807 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PUF4 GO evidence and references
  View Computational GO annotations for PUF4
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 7 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
overexpression
Resources
133 total interaction(s) for 127 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 48
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 3
  • Biochemical Activity: 3
  • Co-crystal Structure: 1
  • PCA: 8
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 50
  • Phenotypic Enhancement: 1
  • Positive Genetic: 9
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 888
Molecular Weight (Da) 97,797
Isoelectric Point (pI) 6.59
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:466141 to 468807 | ORF Map | GBrowse
This feature contains embedded feature(s): YGL014C-A
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2667 466141..468807 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002982
References cited on this page View Complete Literature Guide for PUF4
1) Olivas W and Parker R  (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
2) Chen WN, et al.  (1991) The YGL023 gene encodes a putative regulatory protein. Yeast 7(3):309-12
3) Gerber AP, et al.  (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79