ERG4/YGL012W Summary Help

Standard Name ERG4 1
Systematic Name YGL012W
Feature Type ORF, Verified
Description C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol (2, 3, 4, 5 and see Summary Paragraph)
Name Description ERGosterol biosynthesis 1
Chromosomal Location
ChrVII:472855 to 474276 | ORF Map | GBrowse
Gene Ontology Annotations All ERG4 GO evidence and references
  View Computational GO annotations for ERG4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 12 genes
Classical genetics
Large-scale survey
207 total interaction(s) for 119 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • PCA: 16

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 136
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 29
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 473
Molecular Weight (Da) 56,039
Isoelectric Point (pI) 8.77
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:472855 to 474276 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1422 472855..474276 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002980

ERG4 encodes sterol C-24(28) reductase, which catalyzes the final step in ergosterol biosynthesis (4, 5, 2, 3). Cells lacking ERG4 are viable, but lack ergosterol and are sensitive to several drugs (4, 6, 7). A mutation in ERG4 increases expression of ERG3, which encodes another enzyme involved in ergosterol biosynthesis (8). The S. pombe gene sts1 encodes a protein similar to Erg4p (9).

Last updated: 2000-09-06 Contact SGD

References cited on this page View Complete Literature Guide for ERG4
1) Lai MH, et al.  (1994) The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway. Gene 140(1):41-9
2) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
3) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
4) Zweytick D, et al.  (2000) Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, erg4p, from the yeast saccharomyces cerevisiae. FEBS Lett 470(1):83-7
5) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Sauer B  (1992) Identification of cryptic lox sites in the yeast genome by selection for Cre-mediated chromosome translocations that confer multiple drug resistance. J Mol Biol 223(4):911-28
7) Kaur R and Bachhawat AK  (1999) The yeast multidrug resistance pump, Pdr5p, confers reduced drug resistance in erg mutants of Saccharomyces cerevisiae. Microbiology 145 ( Pt 4)():809-18
8) Arthington-Skaggs BA, et al.  (1996) Positive and negative regulation of a sterol biosynthetic gene (ERG3) in the post-squalene portion of the yeast ergosterol pathway. FEBS Lett 392(2):161-5
9) Shimanuki M, et al.  (1992) Fission yeast sts1+ gene encodes a protein similar to the chicken lamin B receptor and is implicated in pleiotropic drug-sensitivity, divalent cation-sensitivity, and osmoregulation. Mol Biol Cell 3(3):263-73